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ANDRO (Shorten Name),
Product Name:

General ANDRO/ Androstenedione ELISA Kit

 
Cat.#:

E0456Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

4-androstenedione,17-ketoestosterone

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.312-20ng/mL
Sensitivity:
0.078ng/mL
Specificity:
Natural and recombinant general Androstenedione
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0456Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Androstenedione) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Androstenedione. The ELISA analytical biochemical technique of the E0456Ge kit is based on Androstenedione antibody-Androstenedione antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Androstenedione antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Androstenedione. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General ANDRO ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Recovery
Recovery was determined by spiking various levels of Androstenedione into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

96

90-102

Plasma

98

92-104

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Androstenedione and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

89-102%

96-107%

83-93%

81-91%

EDTA plasma(n=5)

97-107%

94-103%

113-125%

87-97%

heparin plasma(n=5)

109-119%

 

85-95%

96-109%

101-111%

 

General Annotation


Sub Unit:
N/A


Function:
Androstenedione (also known as 4-androstenedione and 17-ketoestosterone) is a 19-carbon steroid hormone produced in the adrenal glands and the gonads as an intermediate step in the biochemical pathway that produces the androgen testosterone and the estrogens estrone and estradiol.


Location:
Androstenedione is the common precursor of male and female sex hormones. Some androstenedione is also secreted into the plasma, and may be converted in peripheral tissues to testosterone and estrogens.


Sample Data
Sample Data
Sample Data
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