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E3 (Shorten Name ),
Product Name:

General E3/ Estriol ELISA Kit

Cat.#:

E0455Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

oestriol

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
3.12-200ng/mL
Sensitivity:
1.34ng/mL
Specificity:
Natural and recombinant general Estriol
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
General E3 ELISA Kit
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General E3 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Estriol and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

103-113%

94-107%

109-120%

91-100%

EDTA plasma(n=5)

102-112%

108-117%

83-93%

91-101%

heparin plasma(n=5)

97-109%

 

101-111%

103-113%

106-115%

 

General Annotation


Sub Unit:
N/A


Function:
Estriol (also oestriol) is one of the three main estrogens produced by the human body. Estriol (E3) is only produced in significant amounts during pregnancy as it is made by the placenta from 16-Hydroxydehydroepiandrosterone sulfate (16-OH DHEAS), an androgen steroid made in the fetal liver and adrenal glands.


Location:
The human placenta produces pregnenolone and progesterone from circulating cholesterol. Pregnenolone is converted in the fetal adrenal gland into dehydroepiandrosterone (DHEA), a C19 steroid, then subsequently sulfonated to dehydroepiandrosterone sulfate (DHEAS). DHEAS is converted to 16-OH DHEAS in the fetal liver. The placenta converts 16-OH DHEAS to estriol, and is the predominant site of estriol synthesis.


This product has not yet been referenced specifically in any publications.
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