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E3 (Shorten Name),
Product Name:

General E3/ Estriol ELISA Kit

 
Cat.#:

E0455Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

oestriol

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
3.12-200ng/mL
Sensitivity:
1.34ng/mL
Specificity:
Natural and recombinant general Estriol
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0455Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Estriol) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Estriol. The ELISA analytical biochemical technique of the E0455Ge kit is based on Estriol antibody-Estriol antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Estriol antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Estriol. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General E3 ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Estriol and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

103-113%

94-107%

109-120%

91-100%

EDTA plasma(n=5)

102-112%

108-117%

83-93%

91-101%

heparin plasma(n=5)

97-109%

 

101-111%

103-113%

106-115%

 

General Annotation


Sub Unit:
N/A


Function:
Estriol (also oestriol) is one of the three main estrogens produced by the human body. Estriol (E3) is only produced in significant amounts during pregnancy as it is made by the placenta from 16-Hydroxydehydroepiandrosterone sulfate (16-OH DHEAS), an androgen steroid made in the fetal liver and adrenal glands.


Location:
The human placenta produces pregnenolone and progesterone from circulating cholesterol. Pregnenolone is converted in the fetal adrenal gland into dehydroepiandrosterone (DHEA), a C19 steroid, then subsequently sulfonated to dehydroepiandrosterone sulfate (DHEAS). DHEAS is converted to 16-OH DHEAS in the fetal liver. The placenta converts 16-OH DHEAS to estriol, and is the predominant site of estriol synthesis.


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