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17OHP (Shorten Name),
Product Name:

General 17OHP/ 17-Hydroxyprogesterone ELISA Kit

 
Cat.#:

E0454Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

17-OH progesterone

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
31.2-2000pg/mL
Sensitivity:
10.2pg/mL
Specificity:
Natural and recombinant general 17-Hydroxyprogesterone
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0454Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (17-Hydroxyprogesterone) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 17-Hydroxyprogesterone. The ELISA analytical biochemical technique of the E0454Ge kit is based on 17-Hydroxyprogesterone antibody-17-Hydroxyprogesterone antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 17-Hydroxyprogesterone antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 17-Hydroxyprogesterone. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General 17OHP ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Recovery
Recovery was determined by spiking various levels of 17-Hydroxyprogesterone into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

97

91-103

Plasma

99

93-105

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 17-Hydroxyprogesterone and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

84-95%

107-116%

101-110%

97-107%

EDTA plasma(n=5)

95-104%

107-116%

91-103%

106-116%

heparin plasma(n=5)

96-105%

 

91-100%

107-117%

110-120%

 

General Annotation


Sub Unit:
N/A


Function:
17-Hydroxyprogesterone (17-OH progesterone or 17OHP) is a C-21 steroid hormone produced during the synthesis of glucocorticoids and sex steroids. As a hormone, 17OHP also interacts with the progesterone receptor.


Location:
Measurements of levels of 17-hydroxyprogesterone are useful in the evaluation of patients with suspected congenital adrenal hyperplasia as the typical enzymes that are defective, namely 21-hydroxylase and 11β-hydroxylase, lead to a build-up of 17OHP. In contrast, the rare patient with 17α-hydroxylase deficiency will have very low or undetectable levels of 17OHP. 17OHP levels can also be used to measure contribution of progestational activity of the corpus luteum during pregnancy as progesterone but not 17OHP is also contributed by the placenta.


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