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T4 (Shorten Name),
Product Name:

General T4/ Thyroxine ELISA Kit

 
Cat.#:

E0452Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.195-12.5ng/mL
Sensitivity:
0.0975ng/mL
Specificity:
Natural and recombinant general Thyroxine
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0452Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Thyroxine) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Thyroxine. The ELISA analytical biochemical technique of the E0452Ge kit is based on Thyroxine antibody-Thyroxine antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Thyroxine antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Thyroxine. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General T4 ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Thyroxine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

95-105%

80-89%

92-102%

91-101%

EDTA plasma(n=5)

110-110%

106-115%

91-100%

92-103%

heparin plasma(n=5)

98-108%

 

102-112%

108-118%

104-114%

 

General Annotation


Sub Unit:
N/A


Function:
Thyroxine, or 3,5,3',5'-tetraiodothyronine (often abbreviated as T4), a form of thyroid hormones, is the major hormone secreted by the follicular cells of the thyroid gland.


Location:
Thyroxine is synthesized via the iodination and covalent bonding of the phenyl portions of tyrosine residues found in an initial peptide, thyroglobulin, which is secreted into thyroid granules. These iodinated diphenyl compounds are cleaved from their peptide backbone upon being stimulated by thyroid-stimulating hormone.



[3].
Suyoung Kim, Kyunghee Ji, Sangwoo Lee, Jinyoung Lee, Jungkon Kim, Sunmi Kim, Younglim Kho, Kyungho Choi
Sample Data
Sample Data
Sample Data
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