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T3 (短名 ),

产品名称:

General T3/ Triiodothyronine ELISA Kit
三碘甲状腺原氨酸

货号:

E0453Ge

商标:

EIAab^®

监管等级:

RUO

检测方法:

ELISA

实验类型:

Competitive

检测范围:

78-5000pg/mL

灵敏度:

35pg/mL

特异性:

Natural and recombinant general Triiodothyronine

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

规格 & 价格: 登录.

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General T3 ELISA Kit

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产品说明书

数据表:

说明书:

MSDS:

数据
文献 ( 30 )
序列 / 3D
常见问题解答
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线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	87-97%	92-100%	95-104%	80-91%
EDTA plasma(n=5)	106-114%	103-112%	90-100%	86-95%
heparin plasma(n=5)	110-119%	104-114%	81-91%	90-100%

通用注释

亚单元:

N/A

功能:

Triiodothyronine, C15H12I3NO4, also known as T3, is a thyroid hormone. It affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate.

Location:

The T3 (and T4) bind to nuclear receptors, thyroid receptors. T3 (and T4) are very lipophilic and able to pass through the phospholipid bilayers of target cells. The lipophilicity of T3 (and T4) requires their binding to the protein carrier thyroid-binding protein (TBG) [thyroxine-binding globulins, thyroxine binding prealbumins, and albumins] for transport in the blood. The thyroid receptors bind to response elements in gene promoters, thus enabling them to activate or inhibit transcription. The sensitivity of a tissue to T3 is modulated through the thyroid receptors.

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序列长度:

N/A

序列:

C15H12I3NO | 4

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Parental exposure to tebuconazole causes thyroid endocrine disruption in zebrafish and developmental toxicity in offspring"

SY Li, Q Wu, S Coffin, et al.

From: Aquatic Toxicology

[2].

"Perfluorooctane sulfonic acid exposure increases cadmium toxicity in early life stage of zebrafish, Danio rerio"

Suyoung Kim, Kyunghee Ji, Sangwoo Lee, Jinyoung Lee, Jungkon Kim, Sunmi Kim, Younglim Kho, Kyungho Choi

From: Environmental Toxicology and Chemistry

[3].

"Parental exposure to cadmium chloride causes developmental toxicity and thyroid endocrine disruption in zebrafish offspring"

JJ Tian, J Hu, W He, et al.

From: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology

[4].

"Bioconcentration of 2,4,6-tribromophenol (TBP) and thyroid endocrine disruption in zebrafish larvae"

Jj Fu, Yy Guo, M Wang, et al.

From: Ecotoxicology and Environmental Safety

[5].

"Toxicity and Accumulation of 6-OH-BDE-47 and Newly Synthesized 6,6′-diOH-BDE-47 in Early Life-Stages of Zebrafish (Danio rerio)"

Mt Zhang, F Zhao, Jw Zhang, et al.

From: Science of The Total Environment

[6].

"Waterborne uranium causes toxic effect and thyroid disruption in zebrafish larvae"

C Xu, Ty Li, Cj Hu, et al.

From: Ecotoxicology and Environmental Safety

[7].

"Crosstalk of cholinergic pathway on thyroid disrupting effects of the insecticide chlorpyrifos in zebrafish (Danio rerio)"

K Qiao, Tt Hu, Y Jiang, et al.

From: Science of The Total Environment

[8].

"The joint effect of parental exposure to microcystin-LR and polystyrene nanoplastics on the growth of zebrafish offspring"

Jl Zuo, Tb Huo, X Du, et al.

From: Journal of Hazardous Materials

[9].

"Fiber Specific Changes in Sphingolipid Metabolism in Skeletal Muscles of Hyperthyroid Rats"

A. Chabowski, M. Żendzian-Piotrowska, A. Mikłosz, et al.

From: Lipids

[10].

"Raf-1 is dually down regulated by p,p′-DDE via reduced thyroid hormone and activated ERK"

C Liu, Y Wang, C Quan, M Yan, J Zhou, C Wang, K Yang

From: Pesticide Biochemistry and Physiology

[11].

"Effects of hyperthyroidism on lipid content and composition in oxidative and glycolytic muscles in rats"

A Miklosz, A Chabowski, M Zendzian-Piotrowska

From: Journal of physiology and Pharmacology

[12].

"JNK pathway decreases thyroid hormones via TRH receptor: A novel mechanism for disturbance of thyroid hormone homeostasis by PCB153"

C Liu, M Ha, Y Cui, et al.

From: Toxicology

[13].

"Bioconcentration and metabolism of BDE-209 in the presence of titanium dioxide nanoparticles and impact on the thyroid endocrine system and..."

Q Wang, Q Chen, P Zhou, et al.

From: Nanotoxicology

[14].

"Parental transfer of polybrominated diphenyl ethers (PBDEs) and thyroid endocrine disruption in zebrafish"

L Yu, JCW Lam, Y Guo, et al.

From: Environmental science & technology

[15].

"Chronic exposure to pentachlorophenol alters thyroid hormones and thyroid hormone pathway mRNAs in zebrafish"

LQ Yu, GF Zhao, M Feng, et al.

From: Environmental Toxicology and Chemistry

[16].

"PCB153 and p, p′-DDE disorder thyroid hormones via thyroglobulin, deiodinase 2, transthyretin, hepatic enzymes and receptors"

C Liu, M Ha, L Li, et al.

From: Environ Sci Pollut Res

[17].

"The PI3K/Akt and ERK pathways elevate thyroid hormone receptor β1 and TRH receptor to decrease thyroid hormones after exposure to PCB153 and p,p′-DDE"

C Liu, L LI, M Ha, et al.

From: Chemosphere

[18].

"Waterborne exposure to microcystin-LR alters thyroid hormone levels and gene transcription in the hypothalamic–pituitary–thyroid axis in zebrafish..."

W Yan, Y Zhou, J Yang, et al.

From: Chemosphere

[19].

"Enantioselective thyroid disruption in zebrafish embryo-larvae via exposure to environmental concentrations of the chloroacetamide herbicide acetochlor"

C Xu, X Sun, L Niu, et al.

From: Science of The Total Environment

[20].

"Maternal exposure to fipronil results in sulfone metabolite enrichment and transgenerational toxicity in zebrafish offspring: Indication for an overlooked risk in maternal transfer?"

D Wu, XL Zhi, M Zhang, et al.

From: Environmental Pollution

[21].

"DEHP reduces thyroid hormones via interacting with hormone synthesis-related proteins, deiodinases, transthyretin, receptors, and hepatic enzymes ..."

C Liu, L Zhao, L Wei, et al.

From: Environmental Science and Pollution Research

[22].

"Effects of titanium dioxide nanoparticles on lead bioconcentration and toxicity on thyroid endocrine system and neuronal development in zebrafish ..."

W Miao, B Zhu, X Xiao, et al.

From: Aquatic Toxicology

[23].

"Bioconcentration, metabolism and alterations of thyroid hormones of Tris (1, 3-dichloro-2-propyl) phosphate (TDCPP) in Zebrafish"

Xu T, Wang Q, Shi Q, et al.

From: Environmental toxicology and pharmacology

[24].

"Hyperthyroidism Evokes Myocardial Ceramide Accumulation"

Mikłosz A, Łukaszuk B, Chabowski A, et al.

From: Cellular Physiology and Biochemistry

[25].

"Prenatal transfer of decabromodiphenyl ether (BDE-209) results in disruption of the thyroid system and developmental toxicity in zebrafish offspring"

Zhihua H, Yuefei L, Shenghu Z, et al.

From: Aquatic Toxicology

[26].

"Multiple approaches to assess the effects of F-53B, a Chinese PFOS alternative, on thyroid endocrine disruption at environmentally relevant concentrations"

M Deng, YM Wu, C Xu, et al.

From: Science of The Total Environment

[27].

"Exposure to PFDoA causes disruption of the hypothalamus-pituitary-thyroid axis in zebrafish larvae"

S Zhang, X Guo, S Lu, et al.

From: Environmental Pollution

[28].

"Long-term exposure to the non-steroidal anti-inflammatory drug (NSAID) naproxen causes thyroid disruption in zebrafish at environmentally relevant concentrations"

C Xu, Ll Niu, Hq Guo, et al.

From: Science of The Total Environment

[29].

"Embryonic exposure to soil samples from a gangue stacking area induces thyroid hormone disruption in zebrafish"

Fl Yang, Gk Li, N Sang.

From: Chemosphere

[30].

"Titanium dioxide nanoparticles enhanced thyroid endocrine disruption of pentachlorophenol rather than neurobehavioral defects in zebrafish larvae"

L Lei, K Qiao, J Han, et al.

From: Chemosphere

[1].

通用三碘甲状腺原氨酸(T3)ELISA试剂盒可以做多少个样本？

通用三碘甲状腺原氨酸(T3)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

通用三碘甲状腺原氨酸(T3)ELISA试剂盒使用视频？

通用三碘甲状腺原氨酸(T3)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

通用三碘甲状腺原氨酸(T3)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的通用三碘甲状腺原氨酸(T3)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

通用三碘甲状腺原氨酸(T3)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

通用三碘甲状腺原氨酸(T3)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析通用三碘甲状腺原氨酸(T3)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

通用三碘甲状腺原氨酸(T3)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集通用三碘甲状腺原氨酸(T3)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

通用三碘甲状腺原氨酸(T3)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

通用三碘甲状腺原氨酸(T3)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

通用三碘甲状腺原氨酸(T3)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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General T3/ Triiodothyronine ELISA Kit 三碘甲状腺原氨酸

E0453Ge

General T3/ Triiodothyronine ELISA Kit
三碘甲状腺原氨酸