T3 (Shorten Name),
Product Name:
General T3/ Triiodothyronine ELISA Kit
Cat.#:
E0453Ge
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
78-5000pg/mL
Sensitivity:
35pg/mL
Specificity:
Natural and recombinant general Triiodothyronine
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0453Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Triiodothyronine) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Triiodothyronine. The ELISA analytical biochemical
technique of the E0453Ge kit is based on Triiodothyronine antibody-Triiodothyronine antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Triiodothyronine
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Triiodothyronine. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
More
- Data
- Citations ( 23 )
- Sequence / 3D
- Feedback Wall (0)
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Triiodothyronine and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
87-97% |
92-100% |
95-104% |
80-91% |
|
EDTA plasma(n=5) |
106-114% |
103-112% |
90-100% |
86-95% |
|
heparin plasma(n=5) |
110-119%
|
104-114% |
81-91% |
90-100% |
General Annotation
Sub Unit:
N/A
Function:
Triiodothyronine, C15H12I3NO4, also known as T3, is a thyroid hormone. It affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate.
Location:
The T3 (and T4) bind to nuclear receptors, thyroid receptors. T3 (and T4) are very lipophilic and able to pass through the phospholipid bilayers of target cells. The lipophilicity of T3 (and T4) requires their binding to the protein carrier thyroid-binding protein (TBG) [thyroxine-binding globulins, thyroxine binding prealbumins, and albumins] for transport in the blood. The thyroid receptors bind to response elements in gene promoters, thus enabling them to activate or inhibit transcription. The sensitivity of a tissue to T3 is modulated through the thyroid receptors.
Vaild Sequence:
[2].
Suyoung Kim, Kyunghee Ji, Sangwoo Lee, Jinyoung Lee, Jungkon Kim, Sunmi Kim, Younglim Kho, Kyungho Choi
[4].
C Liu, Y Wang, C Quan, M Yan, J Zhou, C Wang, K Yang
[5].
A Miklosz, A Chabowski, M Zendzian-Piotrowska
[6].
[7].
[8].
L Yu, JCW Lam, Y Guo, et al.
[9].
LQ Yu, GF Zhao, M Feng, et al.
[10].
[11].
[12].
[13].
[14].
[15].
[16].
[17].
[18].
Mikłosz A, Łukaszuk B, Chabowski A, et al.
[20].
[21].
"Exposure to PFDoA causes disruption of the hypothalamus-pituitary-thyroid axis in zebrafish larvae"
S Zhang, X Guo, S Lu, et al.
From:
Environmental Pollution
[22].
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