DHT (Shorten Name),
Product Name:
General DHT/ Dihydrotestosterone ELISA Kit
Cat.#:
E0443Ge
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.156-10ng/mL
Sensitivity:
0.058ng/mL
Specificity:
Natural and recombinant general Dihydrotestosterone
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0443Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Dihydrotestosterone) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Dihydrotestosterone. The ELISA analytical biochemical
technique of the E0443Ge kit is based on Dihydrotestosterone antibody-Dihydrotestosterone antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Dihydrotestosterone
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Dihydrotestosterone. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Dihydrotestosterone and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
101-109% |
108-118% |
81-93% |
96-105% |
|
EDTA plasma(n=5) |
106-118% |
99-108% |
98-109% |
87-97% |
|
heparin plasma(n=5) |
85-95%
|
87-98% |
95-105% |
106-116% |
General Annotation
Sub Unit:
N/A
Function:
Dihydrotestosterone (5α-Dihydrotestosterone, commonly abbreviated to DHT) is an androgen or male sex hormone. The enzyme 5α-reductase synthesises DHT in the prostate, testes, hair follicles, and adrenal glands. This enzyme reduces the 4,5 double-bond of the hormone testosterone.
Location:
N/A
Vaild Sequence:
This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
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operating instructions
Здравствуйте, простите , что пишу не на английском, но я не смогла найти на вашем сайте инструкцию по Дигидротестостерону,catalog№U0443Ge,пожалуйста, если возможно вышлите мне эту инструкцию на английском и на русском языке. Спасибо, с уважением Ольга Ефремова,врач лабораторной диагностики.
Asked by customer
2015-02-02 17:46
2015-02-02 17:46
Dear Customer,
Thank you for your email.
We could just send you the operating instructions in English.You could check it in attachment for reference.
If you have further questions please do not hesitate to get back to us.
Answered by star
2015-02-02 18:05
2015-02-02 18:05
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