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DHEA (Shorten Name),
Product Name:

General DHEA/ Dehydroepiandrosterone ELISA Kit

 
Cat.#:

E0398Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.312-20ng/mL
Sensitivity:
0.091ng/mL
Specificity:
Natural and recombinant general Dehydroepiandrosterone
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0398Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Dehydroepiandrosterone) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Dehydroepiandrosterone. The ELISA analytical biochemical technique of the E0398Ge kit is based on Dehydroepiandrosterone antibody-Dehydroepiandrosterone antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Dehydroepiandrosterone antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Dehydroepiandrosterone. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General DHEA ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Dehydroepiandrosterone and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

103-112%

114-124%

80-92%

90-99%

EDTA plasma(n=5)

100-108%

110-118%

116-126%

96-106%

heparin plasma(n=5)

103-112%

 

95-106%

104-112%

111-120%

 

General Annotation


Sub Unit:
N/A


Function:
5-Dehydroepiandrosterone (5-DHEA) is a 19-carbon endogenous steroid hormone. It is the major secretory steroidal product of the adrenal glands and is also produced by the gonads and the brain. DHEA is the most abundant circulating steroid in humans. DHEA has been implicated in a broad range of biological effects in humans and other mammals. It acts on the androgen receptor both directly and through its metabolites, which include androstenediol and androstenedione, which can undergo further conversion to produce the androgen testosterone and the estrogens, including estrone, estradiol, and estriol. DHEA is also a potent sigma-1 agonist. It is considered a neurosteroid.


Location:
N/A


Sample Data
Sample Data
Sample Data
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