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cholyglycine (Shorten Name),
Product Name:

General cholyglycine ELISA Kit

 
Cat.#:

E0283Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
1.56-100ng/mL
Sensitivity:
0.5ng/mL
Specificity:
Natural and recombinant general cholyglycine
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0283Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (cholyglycine) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing cholyglycine. The ELISA analytical biochemical technique of the E0283Ge kit is based on cholyglycine antibody-cholyglycine antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect cholyglycine antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, cholyglycine. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General cholyglycine ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of cholyglycine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

111-121%

98-106%

106-116%

103-113%

EDTA plasma(n=5)

90-100%

100-112%

98-110%

105-115%

heparin plasma(n=5)

107-116%

 

105-115%

99-109%

97-106%

 

General Annotation


Sub Unit:
N/A


Function:
Serum cholyglycine (Cholyglycine, CG) is a combination of secondary bile acids with glycine conjugated bile acids, in liver cells, cholesterol, after its complex enzymatic reaction, turn into the primary bile acid. Cholic acid (CA) and chenodeoxycholic acid (the CD-CA). Bile acid steroid nucleus three hydroxyl groups (C3, C7, C12), the side chain hydroxyl at the end of the peptide bond and glycine combined with a molecular weight of 462u. CG normal metabolic pathways of the intestine - liver circulation, CG synthesized by hepatocytes, bile canaliculi, bile duct discharged into the gall bladder, along with the bile into the duodenum to help digestion of food. 95% of bile acid reabsorption in the distal ileum, come back to the liver via the portal vein, and uptake by the liver cells to re-use. Exist in the form of protein binding in serum, the total amount of overflow into the systemic circulation is less than 1%. Under normal circumstances, bile acid content in the peripheral blood, leaving little room for normal adults, whether fasting or postprandial serum CG concentration stabilized at a low level. When liver cells are damaged, the liver cell uptake CG capacity decreased, resulting in increased CG content in the blood; cholestasis, liver excretion of bile acids that barriers to reflux of blood circulation increased CG content, but also to the blood CG levels increased. Therefore, the determination of serum glycocholic acid (SCG) by ELISA method is a sensitive indicator to evaluate the function of liver cells and their hepatobiliary material circulation.


Location:
Normal serum cholyglycine: 1.3 ± 0.8mg / L, range 0.4 to 2.98mg / L, and the low limit of the hepatitis diagnosis <3.18mg / L


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