General cyclicAMP/ Cyclic adenosine monophosphate ELISA Kit

Cat.#:

E0003Ge

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

cAMP,cyclic AMP

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.312-20nmol/L

Sensitivity:

0.096nmol/L

Specificity:

Natural and recombinant general Cyclic adenosine monophosphate

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E0003Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Cyclic adenosine monophosphate) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Cyclic adenosine monophosphate. The ELISA analytical biochemical technique of the E0003Ge kit is based on Cyclic adenosine monophosphate antibody-Cyclic adenosine monophosphate antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cyclic adenosine monophosphate antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Cyclic adenosine monophosphate. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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General cyclicAMP ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 4 )
Sequence / 3D
Feedback Wall (0)

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cyclic adenosine monophosphate and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	85-94%	82-91%	110-119%	101-111%
EDTA plasma(n=5)	96-106%	101-110%	100-112%	100-110%
heparin plasma(n=5)	90-99%	103-114%	114-123%	106-116%

General Annotation

Sub Unit:

N/A

Function:

cAMP is a second messenger, used for intracellular signal transduction, such as transferring into cells the effects of hormones like glucagon and adrenaline, which cannot pass through the cell membrane. It is involved in the activation of protein kinases and regulates the effects of adrenaline and glucagon. cAMP also binds to and regulates the function of ion channels such as the HCN channels and a few other cyclic nucleotide-binding proteins such as Epac1.

Location:

cAMP is synthesised from ATP by adenylyl cyclase located on the inner side of the plasma membrane. Adenylyl cyclase is activated by a range of signaling molecules through the activation of adenylyl cyclase stimulatory G (Gs)-protein-coupled receptors and inhibited by agonists of adenylyl cyclase inhibitory G (Gi)-protein-coupled receptors. Liver adenylyl cyclase responds more strongly to glucagon, and muscle adenylyl cyclase responds more strongly to adrenaline. cAMP decomposition into AMP is catalyzed by the enzyme phosphodiesterase.

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