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ANGPT1 (GeneName), Angiopoietin-1 (ProteinName), angp1_human.
Product Name:

Human ANGPT1/ Angiopoietin-1 ELISA Kit

 
Cat.#:

E0008h

Brand:
EIAab®
Regulatory Status:
Alternative:

ANG-1,KIAA0003

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
7.8-500pg/mL
Sensitivity:
3.11 pg/mL
Specificity:
Natural and recombinant human Angiopoietin-1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Cardiovascular
Product Overview:
E0008h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Angiopoietin-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Angiopoietin-1. The ELISA analytical biochemical technique of the E0008h kit is based on Angiopoietin-1 antibody-Angiopoietin-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Angiopoietin-1 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Angiopoietin-1. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human ANGPT1 ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV:  ≤7.8%

Recovery
Recovery was determined by spiking various levels of Angiopoietin-1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

84

80-90

Plasma

86

80-92

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiopoietin-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

99-107%

98-108%

100-109%

103-112%

EDTA plasma(n=5)

114-124%

95-106%

94-104%

106-114%

heparin plasma(n=5)

105-115%

 

106-117%

93-103%

90-103%

 

General Annotation


Sub Unit:
Homooligomer. Interacts with TEK/TIE2.


Function:
Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.


Subcellular Location:
Secreted



[1].
Yesildal F, Koc E, Tas A, et al.

[1].
"A functional variant in the 3'-UTR of angiopoietin-1 might reduce stroke risk by interfering with the binding efficiency of microRNA 211."

[2].
"Biological characterization of angiopoietin-3 and angiopoietin-4."

[3].
"Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning."

[4].
"Effects of angiopoietins-1 and -2 on the receptor tyrosine kinase Tie2 are differentially regulated at the endothelial cell surface."

[5].
"Hedgehog promotes neovascularization in pancreatic cancers by regulating Ang-1 and IGF-1 expression in bone-marrow derived pro-angiogenic cells."
Sample Data
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