首页 > 重组蛋白 > Human YTHDF2 Recombinant Protein

YTHDF2 (基因名), YTH domain-containing family protein 2 (蛋白名), ythd2_human.

产品名称:

Human YTHDF2/ YTH domain-containing family protein 2 Recombinant Protein

货号:

R3687h

商标:

EIAab^®

监管等级:

RUO

别名:

CLL-associated antigen KW-14, High-glucose-regulated protein 8, Renal carcinoma antigen NY-REN-2, HGRG8

序列号:

Q9Y5A9

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Epigenetics

规格 & 价格:

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Human YTHDF2 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 1p35.3 by HGNC 1p35.3 by Entrez Gene 1p35.3 by Ensembl

YTHDF2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

N/A

功能:

Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability (PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:22575960, PubMed:24284625, PubMed:25412658, PubMed:25412661). Acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation (PubMed:24284625, PubMed:26046440). Plays a key role in maternal-to-zygotic transition during early embryonic development, the process during which maternally inherited mRNAs are degraded: acts by binding m6A-containing maternal mRNAs and promoting their degradation (By similarity). Also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation (PubMed:26458103).

亚细胞位置:

Cytoplasm Cytosol Nucleus Cytoplasm P-body Localizes to the cytosol and relocates to the nucleus following heat shock stress (PubMed:26458103).

数据库链接

UniGene:

Hs.532286

SMR:

Q9Y5A9

STRING:

9606.ENSP00000362918

KEGG:

hsa:51441

MIM:

610640

Pfam:

PF04146

Uniprot:

Q9Y5A9

相关产品

Mouse Ythdf2 ELISA Kit

Bovine YTHDF2 ELISA Kit

Mouse Ythdf2 CLIA Kit

Bovine YTHDF2 CLIA Kit

Mouse Ythdf2 Protein

序列长度:

579

序列:

仅供参考，详细请咨询（services@eiaab.com）

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579:

TSIFDDFSHY | EKRQEEEESV | KKERQGRGK

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq."

(PMID: 22575960)

[2].

"Antigens recognized by autologous antibody in patients with renal-cell carcinoma."

(PMID: 10508479)

[3].

"YTHDF3 facilitates translation and decay of N6-methyladenosine-modified RNA."

(PMID: 28106072)

[4].

"N(6)-methyladenosine Modulates Messenger RNA Translation Efficiency."

(PMID: 26046440)

[5].

"Structural Basis for the Discriminative Recognition of N6-Methyladenosine RNA by the Human YT521-B Homology Domain Family of Proteins."

(PMID: 26318451)

[6].

"Dynamic m(6)A mRNA methylation directs translational control of heat shock response."

(PMID: 26458103)

[7].

"N6-methyladenosine-dependent regulation of messenger RNA stability."

(PMID: 24284625)

[8].

"Structure of the YTH domain of human YTHDF2 in complex with an m(6)A mononucleotide reveals an aromatic cage for m(6)A recognition."

(PMID: 25412658)

[9].

"Crystal structure of the YTH domain of YTHDF2 reveals mechanism for recognition of N6-methyladenosine."

(PMID: 25412661)

[10].

"A polymorphism of the YTHDF2 gene (1p35) located in an Alu-rich genomic domain is associated with human longevity."

(PMID: 16799135)

[1].

人YTHDF2重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人YTHDF2重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人YTHDF2重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人YTHDF2重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人YTHDF2重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人YTHDF2重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人YTHDF2重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人YTHDF2重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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