首页 > 重组蛋白 > Human YAP1 Recombinant Protein

YAP1 (基因名), Transcriptional coactivator YAP1 (蛋白名), yap1_human.

产品名称:

Human YAP1/ Transcriptional coactivator YAP1 Recombinant Protein
约克同源

货号:

R15210h

商标:

EIAab^®

监管等级:

RUO

别名:

Protein yorkie homolog, Yes-associated protein YAP65 homolog, Yes-associated protein 1, YAP65

序列号:

P46937

来源:

E.coli

种属:

Human

标签:

His

序列:

251-400aa

预估分子量:

16.5 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

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Human YAP1 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human YAP1 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 11q22.1 by HGNC 11q22.1 by Entrez Gene 11q22.1 by Ensembl

YAP1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Binds to the SH3 domain of the YES kinase. Binds to WBP1 and WBP2 (PubMed:9202023). Binds, in vitro, through the WW1 domain, to neural isoforms of ENAH that contain the PPSY motif (By similarity). The phosphorylated form interacts with YWHAB (PubMed:17974916). Interacts (via WW domains) with LATS1 (via PPxY motif 2) (PubMed:18158288). Interacts with LATS2 (PubMed:18158288). Isoform 2 and isoform 3 interact (via WW domain 1) with isoform 3 of ERBB4 (via PPxY motif 2) (PubMed:12807903). Interacts with TEAD1, TEAD2, TEAD3 and TEAD4 (PubMed:18579750, PubMed:20123905, PubMed:20123908). Interacts with TP73 (PubMed:18280240). Interacts with RUNX1 (PubMed:18280240). Interacts with HCK (PubMed:17535448). Interacts (via WW domains) with PTPN14 (via PPxY motif 2); this interaction leads to the cytoplasmic sequestration of YAP1 and inhibits its transcriptional coactivator activity (PubMed:22525271).

功能:

Isoform 2: Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).

亚细胞位置:

Cytoplasm Nucleus Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density (PubMed:18158288, PubMed:20048001). PTPN14 induces translocation from the nucleus to the cytoplasm (PubMed:22525271).

数据库链接

UniGene:

Hs.503692

SMR:

P46937

STRING:

9606.ENSP00000282441

KEGG:

hsa:10413

MIM:

120433

Pfam:

PF00397

Uniprot:

P46937

相关产品

Mouse Yap1 ELISA Kit

Rat Yap1 ELISA Kit

Chicken YAP1 ELISA Kit

Mouse Yap1 CLIA Kit

Rat Yap1 CLIA Kit

序列长度:

504

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

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350:

400:

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500:

504:

LTWL

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Characterization of the mammalian YAP (Yes-associated protein) gene and its role in defining a novel protein module, the WW domain."

(PMID: 7782338)

[2].

"Structural insights into the YAP and TEAD complex."

(PMID: 20123905)

[3].

"A coordinated phosphorylation by Lats and CK1 regulates YAP stability through SCF(beta-TRCP)."

(PMID: 20048001)

[4].

"Inactivation of YAP oncoprotein by the Hippo pathway is involved in cell contact inhibition and tissue growth control."

(PMID: 17974916)

[5].

"WW domain-containing protein YAP associates with ErbB-4 and acts as a co-transcriptional activator for the carboxyl-terminal fragment of ErbB-4 that translocates to the nucleus."

(PMID: 12807903)

[6].

"Loss of DLG5 promotes breast cancer malignancy by inhibiting the Hippo signaling pathway."

(PMID: 28169360)

[7].

"Heterozygous loss-of-function mutations in YAP1 cause both isolated and syndromic optic fissure closure defects."

(PMID: 24462371)

[8].

"PTPN14 interacts with and negatively regulates the oncogenic function of YAP."

(PMID: 22525271)

[9].

"Identification, basic characterization and evolutionary analysis of differentially spliced mRNA isoforms of human YAP1 gene."

(PMID: 22939869)

[10].

"Genome-wide association with MRI atrophy measures as a quantitative trait locus for Alzheimer's disease."

(PMID: 21116278)

[1].

人约克同源(YAP1)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人约克同源(YAP1)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人约克同源(YAP1)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人约克同源(YAP1)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人约克同源(YAP1)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人约克同源(YAP1)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人约克同源(YAP1)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人约克同源(YAP1)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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