首页 > 重组蛋白 > Human WDR48 Recombinant Protein

WDR48 (基因名), WD repeat-containing protein 48 (蛋白名), wdr48_human.

产品名称:

Human WDR48/ WD repeat-containing protein 48 Recombinant Protein

货号:

R3788h

商标:

EIAab^®

监管等级:

RUO

别名:

USP1-associated factor 1, WD repeat endosomal protein, p80, KIAA1449, UAF1

序列号:

Q8TAF3

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

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Human WDR48 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 3p22.2 by HGNC 3p22.2 by Entrez Gene 3p22.2 by Ensembl

WDR48 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with USP46 (PubMed:19075014, PubMed:26388029). Interacts with USP1 (PubMed:18082604, PubMed:26388029). Interacts with USP12 (PubMed:19075014, PubMed:27650958, PubMed:27373336). Component of the USP12/WDR20/WDR48 deubiquitinating complex (PubMed:27373336). Interacts with PHLPP1 (PubMed:24145035). Interacts with Saimiriine herpesvirus TIP protein (PubMed:12196293, PubMed:12885920). Interacts with papillomavirus HPV11 E1 protein (PubMed:18032488).

功能:

Regulator of deubiquitinating complexes. Acts as a strong activator of USP1 and USP46 (PubMed:18082604, PubMed:19075014, PubMed:26388029). Enhances the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself (PubMed:18082604). Also activates deubiquitinating activity of complexes containing USP12 (PubMed:19075014, PubMed:27650958, PubMed:27373336). Docks at the distal end of the USP12 fingers domain and induces a cascade of structural changes leading to the activation of the enzyme (PubMed:27650958, PubMed:27373336). Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate (PubMed:19075014, PubMed:27373336). In complex with USP12, acts as a potential tumor suppressor by positively regulating PHLPP1 stability (PubMed:24145035). In case of infection by Herpesvirus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP (PubMed:12196293). In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1 (PubMed:18032488).

亚细胞位置:

Nucleus Cytoplasm Lysosome Late endosome Mainly in cytoplasmic compartments (PubMed:12196293, PubMed:18032488). In case of infection by papillomavirus HPV11, translocates to the nucleus via its interaction with papillomavirus HPV11 (PubMed:18032488).

数据库链接

UniGene:

Hs.109778

SMR:

Q8TAF3

STRING:

9606.ENSP00000307491

KEGG:

hsa:57599

MIM:

612167

Pfam:

PF11816

Uniprot:

Q8TAF3

相关产品

Mouse Wdr48 ELISA Kit

Bovine WDR48 ELISA Kit

Chicken WDR48 ELISA Kit

Mouse Wdr48 CLIA Kit

Bovine WDR48 CLIA Kit

序列长度:

677

序列:

仅供参考，详细请咨询（services@eiaab.com）

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677:

NMDLRTVKHF | IWKSGGDLTL | HYRQKST

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Exome sequencing links corticospinal motor neuron disease to common neurodegenerative disorders."

(PMID: 24482476)

[2].

"Human papillomavirus E1 helicase interacts with the WD repeat protein p80 to promote maintenance of the viral genome in keratinocytes."

(PMID: 18032488)

[3].

"Herpesviral protein targets a cellular WD repeat endosomal protein to downregulate T lymphocyte receptor expression."

(PMID: 12196293)

[4].

"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."

(PMID: 10819331)

[5].

"Allosteric Activation of Ubiquitin-Specific Proteases by β-Propeller Proteins UAF1 and WDR20."

(PMID: 27373336)

[6].

"A conserved two-step binding for the UAF1 regulator to the USP12 deubiquitinating enzyme."

(PMID: 27650958)

[7].

"Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46."

(PMID: 26388029)

[8].

"WD repeat protein WDR48 in complex with deubiquitinase USP12 suppresses Akt-dependent cell survival signaling by stabilizing PH domain leucine-rich repeat protein phosphatase 1 (PHLPP1)."

(PMID: 24145035)

[9].

"UAF1 is a subunit of multiple deubiquitinating enzyme complexes."

(PMID: 19075014)

[10].

"A UAF1-containing multisubunit protein complex regulates the Fanconi anemia pathway."

(PMID: 18082604)

[1].

人WDR48重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人WDR48重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人WDR48重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人WDR48重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人WDR48重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人WDR48重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人WDR48重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人WDR48重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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