首页 > 重组蛋白 > Human WASHC1 Recombinant Protein

WASHC1 (基因名), WASH complex subunit 1 (蛋白名), wash1_human.

产品名称:

Human WASHC1/ WASH complex subunit 1 Recombinant Protein

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

CXYorf1-like protein on chromosome 9, Protein FAM39E, WAS protein family homolog 1, FAM39E, WASH1

序列号:

A8K0Z3

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

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Human WASHC1 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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基因位点

Cytogenetic band: 9p24.3 by HGNC 9p24.3 by Entrez Gene 9p24.3 by Ensembl

WASHC1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Component of the WASH core complex also described as WASH regulatory complex (SHRC) composed of WASH (WASHC1, WASH2P or WASH3P), WASHC2 (WASHC2A or WASHC2C), WASHC3, WASHC4 and WASHC5. The WASH core complex associates via WASHC2 with the F-actin-capping protein dimer (formed by CAPZA1, CAPZA2 or CAPZA3 and CAPZB) in a transient or substoichiometric manner which was initially described as WASH complex (PubMed:19922875, PubMed:20498093). Interacts (via WHD1 region) with WASHC2C; the interaction is direct (PubMed:19922874). Interacts with VPS35; mediates the association with the retromer CSC complex. Interacts with FKBP15. Interacts with alpha-tubulin. Interacts with BECN1; WASHC1 and AMBRA1 can competetively interact with BECN1. Interacts with BLOC1S2; may associate with the BLOC-1 complex. Interacts with tubulin gamma chain (TUBG1 or TUBG2) (By similarity). Interacts with EXOC1, EXOC4, EXOC8; in MMP14-positive endosomes in breast tumor cells; indicative for an association with the exocyst complex (PubMed:24344185).

功能:

Acts as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922874, PubMed:19922875, PubMed:20498093, PubMed:23452853). Its assembly in the WASH core complex seems to inhibit its NPF activity and via WASHC2 is required for its membrane targeting (PubMed:20498093). Involved in endocytic trafficking of EGF (By similarity). Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration (PubMed:22114305). In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation (By similarity). Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex (PubMed:24344185). Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity.

亚细胞位置:

Early endosome membrane Recycling endosome membrane Late endosome Cytoplasmic vesicle Autophagosome Cytoplasm Cytoskeleton Microtubule organizing center Centrosome Centriole Localization to the endosome membrane is mediated via its interaction with WASHC2 (PubMed:19922874). Localizes to MMP14-positive late endosomes and transiently to invadipodia (PubMed:24344185). Localized to Salmonella typhimurium entry sites (By similarity).

数据库链接

UniGene:

Hs.446466

STRING:

9606.ENSP00000352498

KEGG:

hsa:100287171

MIM:

613632

Pfam:

PF11945

Uniprot:

A8K0Z3

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序列长度:

465

序列:

仅供参考，详细请咨询（services@eiaab.com）

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PQQPQAEEDE | DDWES

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Human subtelomeric WASH genes encode a new subclass of the WASP family."

(PMID: 18159949)

[2].

"Regulation of WASH-dependent actin polymerization and protein trafficking by ubiquitination."

(PMID: 23452853)

[3].

"Endosomal WASH and exocyst complexes control exocytosis of MT1-MMP at invadopodia."

(PMID: 24344185)

[4].

"The Arp2/3 activator WASH regulates α5β1-integrin-mediated invasive migration."

(PMID: 22114305)

[5].

"A FAM21-containing WASH complex regulates retromer-dependent sorting."

(PMID: 19922874)

[6].

"The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex."

(PMID: 19922875)

[7].

"Diverse fates of paralogs following segmental duplication of telomeric genes."

(PMID: 15233989)

[8].

"Complete sequencing and characterization of 21,243 full-length human cDNAs."

(PMID: 14702039)

[9].

"Differentially regulated and evolved genes in the fully sequenced Xq/Yq pseudoautosomal region."

(PMID: 10655549)

[10].

"HRS-WASH axis governs actin-mediated endosomal recycling and cell invasion."

(PMID: 29891722)

[1].

人WASHC1重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人WASHC1重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人WASHC1重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人WASHC1重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人WASHC1重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人WASHC1重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人WASHC1重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人WASHC1重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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