首页 > 重组蛋白 > Human WASF1 Recombinant Protein

WASF1 (基因名), Wiskott-Aldrich syndrome protein family member 1 (蛋白名), wasf1_human.

产品名称:

Human WASF1/ Wiskott-Aldrich syndrome protein family member 1 Recombinant Protein

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

Protein WAVE-1, Verprolin homology domain-containing protein 1, WASP family protein member 1, KIAA0269, SCAR1, WAVE1

序列号:

Q92558

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

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Human WASF1 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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基因位点

Cytogenetic band: 6q21 by HGNC 6q21 by Entrez Gene 6q21 by Ensembl

WASF1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Interacts with SHANK3; the interaction mediates the association of SHANK3 with the WAVE1 complex. Interacts with ABI1 (via N-terminus). Interacts with SORBS2; this interaction greatly enhances phosphorylation by ABL1 and dephosphorylation by PTPN12 and might mediate partial to focal adhesion sites.

功能:

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.

亚细胞位置:

Cytoplasm Cytoskeleton Cell junction Synapse Cell junction Focal adhesion Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas (PubMed:9889097). Partial translocation to focal adhesion sites might be mediated by interaction with SORBS2 (PubMed:18559503). In neurons, colocalizes with activated NTRK2 after BDNF addition in endocytic sites through the association with TMEM108 (By similarity).

数据库链接

UniGene:

Hs.75850

SMR:

Q92558

STRING:

9606.ENSP00000352425

KEGG:

hsa:8936

MIM:

605035

Pfam:

PF02205

Uniprot:

Q92558

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序列长度:

559

序列:

仅供参考，详细请咨询（services@eiaab.com）

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EFDEVDWLE

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Scar1 and the related Wiskott-Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex."

(PMID: 9889097)

[2].

"WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac."

(PMID: 9843499)

[3].

"Structure and control of the actin regulatory WAVE complex."

(PMID: 21107423)

[4].

"[Interaction of WAVE1 and genes involved in multiple drug resistance in children with acute myeloblastic leukemia]."

(PMID: 20426950)

[5].

"[Role of WAVE1 in K562 leukemia cells invasion and its mechanism]."

(PMID: 19731823)

[6].

"ArgBP2-dependent signaling regulates pancreatic cell migration, adhesion, and tumorigenicity."

(PMID: 18559503)

[7].

"WAVE1 controls neuronal activity-induced mitochondrial distribution in dendritic spines."

(PMID: 18287015)

[8].

"[Expression of WAVE1 in childhood acute lymphocytic leukemia and in the apoptosis of Jurkat cells induced by adriamycin]."

(PMID: 18947485)

[9].

"WAVE/Scars in platelets."

(PMID: 15280206)

[10].

"Expression and subcellular localization of WAVE isoforms in the megakaryocyte/platelet lineage."

(PMID: 15670045)

[1].

人WASF1重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人WASF1重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人WASF1重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人WASF1重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人WASF1重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人WASF1重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人WASF1重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人WASF1重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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