首页 > 重组蛋白 > Human VHL Recombinant Protein

VHL (基因名), Von Hippel-Lindau disease tumor suppressor (蛋白名), vhl_human.

产品名称:

Human VHL/ Von Hippel-Lindau disease tumor suppressor Recombinant Protein
HumanVonHippel-Lindaudiseasetumorsuppressor

货号:

R1894h

商标:

EIAab^®

监管等级:

RUO

别名:

Protein G7, pVHL

序列号:

P40337

来源:

E.coli

种属:

Human

标签:

His

序列:

1-213aa

预估分子量:

23.43 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

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产品说明书

说明书:

MSDS:

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数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human VHL protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 3p25.3 by HGNC 3p25.3 by Entrez Gene 3p25.3 by Ensembl

VHL Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Component of the VCB (VHL-Elongin BC-CUL2) complex; this complex acts as a ubiquitin-ligase E3 and directs proteasome-dependent degradation of targeted proteins. Interacts with CUL2; this interaction is dependent on the integrity of the trimeric VBC complex. Interacts (via the beta domain) with HIF1A (via the NTAD domain); this interaction mediates degradation of HIF1A in normoxia and, in hypoxia, prevents ubiquitination and degradation of HIF1A by mediating hypoxia-induced translocation to the nucleus, a process which requires a hypoxia-dependent regulatory signal. Interacts with ADRB2; the interaction, in normoxia, is dependent on hydroxylation of ADRB2 and the subsequent VCB-mediated ubiquitination and degradation of ADRB2. Under hypoxia, hydroxylation, interaction with VHL, ubiquitination and subsequent degradation of ADRB2 are dramatically decreased. Interacts with RNF139, USP33 and JADE1. Found in a complex composed of LIMD1, VHL, EGLN1/PHD2, ELOB and CUL2. Isoform 1 and isoform 3 interact with LIMD1 (via LIM zinc-binding 2), AJUBA (via LIM domains) and WTIP (via LIM domains). Interacts with EPAS1. Interacts with CARD9.

功能:

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2.

亚细胞位置:

Isoform 3 Cytoplasm Nucleus Equally distributed between the nucleus and the cytoplasm but not membrane-associated.

数据库链接

UniGene:

Hs.517792

SMR:

P40337

STRING:

9606.ENSP00000256474

KEGG:

hsa:7428

MIM:

144700

Pfam:

PF01847

Uniprot:

P40337

相关产品

序列长度:

213

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

213:

LTQERIAHQR | MGD

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Mutations of von Hippel-Lindau tumor-suppressor gene and congenital polycythemia."

(PMID: 12844285)

[2].

"A second major native von Hippel-Lindau gene product, initiated from an internal translation start site, functions as a tumor suppressor."

(PMID: 9671762)

[3].

"TP53, EGFR, and KRAS mutations in relation to VHL inactivation and lifestyle risk factors in renal-cell carcinoma from central and eastern Europe."

(PMID: 20137853)

[4].

"Oxygen-regulated beta(2)-adrenergic receptor hydroxylation by EGLN3 and ubiquitylation by pVHL."

(PMID: 19584355)

[5].

"Head and neck paragangliomas in von Hippel-Lindau disease and multiple endocrine neoplasia type 2."

(PMID: 19336503)

[6].

"Hypertension, antihypertensives and mutations in the Von Hippel-Lindau gene in renal cell carcinoma: results from the Netherlands Cohort Study."

(PMID: 16208141)

[7].

"Congenital disorder of oxygen sensing: association of the homozygous Chuvash polycythemia VHL mutation with thrombosis and vascular abnormalities but not tumors."

(PMID: 14726398)

[8].

"Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein."

(PMID: 11739384)

[9].

"The von Hippel-Lindau tumor suppressor stabilizes novel plant homeodomain protein Jade-1."

(PMID: 12169691)

[10].

"Germ-line mutations in nonsyndromic pheochromocytoma."

(PMID: 12000816)

[1].

人HumanVonHippel-Lindaudiseasetumorsuppressor(VHL)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人HumanVonHippel-Lindaudiseasetumorsuppressor(VHL)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人HumanVonHippel-Lindaudiseasetumorsuppressor(VHL)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人HumanVonHippel-Lindaudiseasetumorsuppressor(VHL)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人HumanVonHippel-Lindaudiseasetumorsuppressor(VHL)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人HumanVonHippel-Lindaudiseasetumorsuppressor(VHL)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人HumanVonHippel-Lindaudiseasetumorsuppressor(VHL)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人HumanVonHippel-Lindaudiseasetumorsuppressor(VHL)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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