首页 > 重组蛋白 > Human TEC Recombinant Protein

TEC (基因名), Tyrosine-protein kinase Tec (蛋白名), tec_human.

产品名称:

Human TEC/ Tyrosine-protein kinase Tec Recombinant Protein
酪氨酸蛋白激酶Tec

货号:

R8704h

商标:

EIAab^®

监管等级:

RUO

别名:

PSCTK4

序列号:

P42680

来源:

E.coli

种属:

Human

标签:

His

序列:

101-310aa

预估分子量:

23.1 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Signal Transduction

规格 & 价格:

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Human TEC Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human TEC protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 4p12-p11 by HGNC 4p12-p11 by Entrez Gene 4p12-p11 by Ensembl

TEC Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with INPP5D/SHIP1 and INPPL1/SHIP2. Interacts with CD28, FASLG, FGF2, GRB10, LYN and KIT. Interacts with VAV1 and JAK2.

功能:

Non-receptor tyrosine kinase that contributes to signaling from many receptors and participates as a signal transducer in multiple downstream pathways, including regulation of the actin cytoskeleton. Plays a redundant role to ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Required for TCR-dependent IL2 gene induction. Phosphorylates DOK1, one CD28-specific substrate, and contributes to CD28-signaling. Mediates signals that negatively regulate IL2RA expression induced by TCR cross-linking. Plays a redundant role to BTK in BCR-signaling for B-cell development and activation, especially by phosphorylating STAP1, a BCR-signaling protein. Required in mast cells for efficient cytokine production. Involved in both growth and differentiation mechanisms of myeloid cells through activation by the granulocyte colony-stimulating factor CSF3, a critical cytokine to promoting the growth, differentiation, and functional activation of myeloid cells. Participates in platelet signaling downstream of integrin activation. Cooperates with JAK2 through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. GRB10, a negative modifier of the FOS activation pathway, is another substrate of TEC. TEC is involved in G protein-coupled receptor- and integrin-mediated signalings in blood platelets. Plays a role in hepatocyte proliferation and liver regeneration and is involved in HGF-induced ERK signaling pathway. TEC regulates also FGF2 unconventional secretion (endoplasmic reticulum (ER)/Golgi-independent mechanism) under various physiological conditions through phosphorylation of FGF2 'Tyr-215'. May also be involved in the regulation of osteoclast differentiation.

亚细胞位置:

Cytoplasm Cell membrane Peripheral membrane protein Cytoplasm Cytoskeleton Following B-cell or T-cell receptors activation by antigen, translocates to the plasma membrane through its PH domain. Thrombin and integrin engagement induces translocation of TEC to the cytoskeleton during platelet activation. In cardiac myocytes, assumes a diffuse intracellular localization under basal conditions but is recruited to striated structures upon various stimuli, including ATP (By similarity).

数据库链接

UniGene:

Hs.479670

SMR:

P42680

STRING:

9606.ENSP00000370912

KEGG:

hsa:7006

MIM:

600583

Pfam:

PF00779

Uniprot:

P42680

相关产品

Mouse Tec ELISA Kit

Mouse Tec CLIA Kit

Mouse Tec Protein

Mouse Tec Polyclonal Antibody

Mouse Tec Monoclonal Antibody

序列长度:

631

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

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631:

CWQEKPEGRP | SFEDLLRTID | ELVECEETFG | R

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Molecular cloning and analysis of the human Tec protein-tyrosine kinase."

(PMID: 7934162)

[2].

"Tec protein tyrosine kinase inhibits CD25 expression in human T-lymphocyte."

(PMID: 19883687)

[3].

"SHIP family inositol phosphatases interact with and negatively regulate the Tec tyrosine kinase."

(PMID: 15492005)

[4].

"Identification of phosphorylation sites within the SH3 domains of Tec family tyrosine kinases."

(PMID: 12573241)

[5].

"Molecular cloning of a docking protein, BRDG1, that acts downstream of the Tec tyrosine kinase."

(PMID: 10518561)

[6].

"Tec is involved in G protein-coupled receptor- and integrin-mediated signalings in human blood platelets."

(PMID: 9652744)

[7].

"Integrin-dependent tyrosine phoshorylation and cytoskeletal translocation of Tec in thrombin-activated platelets."

(PMID: 9299487)

[8].

"Tec kinase associates with c-kit and is tyrosine phosphorylated and activated following stem cell factor binding."

(PMID: 7526158)

[9].

"Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score."

(PMID: 20379614)

[10].

"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

(PMID: 20628086)

[1].

人酪氨酸蛋白激酶Tec(TEC)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人酪氨酸蛋白激酶Tec(TEC)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人酪氨酸蛋白激酶Tec(TEC)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人酪氨酸蛋白激酶Tec(TEC)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人酪氨酸蛋白激酶Tec(TEC)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人酪氨酸蛋白激酶Tec(TEC)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人酪氨酸蛋白激酶Tec(TEC)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人酪氨酸蛋白激酶Tec(TEC)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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