首页 > 重组蛋白 > Human SNCA Recombinant Protein

SNCA (基因名), Alpha-synuclein (蛋白名), syua_human.

产品名称:

Human SNCA/ Alpha-synuclein Recombinant Protein
α突触核蛋白

货号:

R1222h

商标:

EIAab^®

监管等级:

RUO

别名:

Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, NACP, PARK1

序列号:

P37840

来源:

E.coli

种属:

Human

标签:

His

序列:

1-140aa

预估分子量:

15.4 kDa (monomer)

纯度:

Greater than 95% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Neurosciences

规格 & 价格:

关注

Human SNCA Protein

邮箱 *

消息 *

Please 登录.

产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
反馈墙 (0)

R&D 技术数据

The PCR product of human SNCA gene was determined by 1% Agarose stained with EB.

Recombinant human SNCA protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 4q22.1 by HGNC 4q22.1 by Entrez Gene 4q22.1 by Ensembl

SNCA Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Soluble monomer. Homotetramer (PubMed:21841800). A dynamic intracellular population of tetramers and monomers coexists normally and the tetramer plays an essential role in maintaining homeostasis (PubMed:21841800). Interacts with UCHL1 (By similarity). Interacts with phospholipase D and histones. Interacts (via N-terminus) with synphilin-1/SNCAIP; this interaction promotes formation of SNCA inclusions in the cytoplasm (PubMed:19762560). Interacts with CALM1 (PubMed:23607618). Interacts with STXBP1; this interaction controls SNCA self-replicating aggregation (PubMed:27597756). Interacts with SNARE components VAMP2 and SNAP25; these interactions allows SNARE complex assembly and integrity (PubMed:20798282). Interacts with RPH3A and RAB3A (PubMed:15207266).

功能:

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).

亚细胞位置:

Cytoplasm Membrane Nucleus Cell junction Synapse Secreted Membrane-bound in dopaminergic neurons.

数据库链接

UniGene:

Hs.21374

SMR:

P37840

STRING:

9606.ENSP00000338345

KEGG:

hsa:6622

MIM:

127750

Pfam:

PF01387

Uniprot:

P37840

相关产品

Mouse Snca ELISA Kit

Rat Snca ELISA Kit

Bovine SNCA ELISA Kit

Pig SNCA ELISA Kit

Mouse Snca CLIA Kit

序列长度:

140

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

142:

LGKNEEGAPQ | EGILEDMPVD | P DNEAYEMP | SEEGYQDYEP | EA

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Genetic association studies of methamphetamine use disorders: A systematic review and synthesis."

(PMID: 19219857)

[2].

"Alpha-synuclein gene rearrangements in dominantly inherited parkinsonism: frequency, phenotype, and mechanisms."

(PMID: 19139307)

[3].

"Lipid rafts mediate the synaptic localization of alpha-synuclein."

(PMID: 15282274)

[4].

"Mutation E46K increases phospholipid binding and assembly into filaments of human alpha-synuclein."

(PMID: 15498564)

[5].

"Study of association between alpha-synuclein gene polymorphism and methamphetamine psychosis/dependence."

(PMID: 15542733)

[1].

人α突触核蛋白(SNCA)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人α突触核蛋白(SNCA)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人α突触核蛋白(SNCA)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人α突触核蛋白(SNCA)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人α突触核蛋白(SNCA)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人α突触核蛋白(SNCA)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人α突触核蛋白(SNCA)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人α突触核蛋白(SNCA)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

反馈墙

评论数 : 0

所有用户