首页 > 重组蛋白 > Human SGPP1 Recombinant Protein

SGPP1 (基因名), Sphingosine-1-phosphate phosphatase 1 (蛋白名), sgpp1_human.

产品名称:

Human SGPP1/ Sphingosine-1-phosphate phosphatase 1 Recombinant Protein
磷酸鞘氨醇磷酸酶1

货号:

R11211h

商标:

EIAab^®

监管等级:

RUO

别名:

Sphingosine-1-phosphatase 1, Sphingosine-1-phosphate phosphohydrolase 1, SPP-1, SPPase1, SPP1

序列号:

Q9BX95

来源:

E.coli

种属:

Human

标签:

His

序列:

1-131aa

预估分子量:

14.41 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

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Human SGPP1 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human SGPP1 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 14q23.2 by HGNC 14q23.2 by Entrez Gene 14q23.2 by Ensembl

SGPP1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

N/A

功能:

Specifically dephosphorylates sphingosine 1-phosphate (S1P), dihydro-S1P, and phyto-S1P. Does not act on ceramide 1-phosphate, lysophosphatidic acid or phosphatidic acid (PubMed:16782891). Sphingosine-1-phosphate phosphatase activity is needed for efficient recycling of sphingosine into the sphingolipid synthesis pathway (PubMed:12815058, PubMed:11756451, PubMed:16782891). Regulates the intracellular levels of the bioactive sphingolipid metabolite S1P that regulates diverse biological processes acting both as an extracellular receptor ligand or as an intracellular second messenger (PubMed:11756451, PubMed:12815058, PubMed:16782891). Involved in efficient ceramide synthesis from exogenous sphingoid bases. Converts S1P to sphingosine, which is readily metabolized to ceramide via ceramide synthase. In concert with sphingosine kinase 2 (SphK2), recycles sphingosine into ceramide through a phosphorylation/dephosphorylation cycle (By similarity). Regulates endoplasmic-to-Golgi trafficking of ceramides, resulting in the regulation of ceramide levels in the endoplasmic reticulum, preferentially long-chain ceramide species, and influences the anterograde membrane transport of both ceramide and proteins from the endoplasmic retiulum to the Golgi apparatus (PubMed:16782891). The modulation of intracellular ceramide levels in turn regulates apoptosis (By similarity). Via S1P levels, modulates resting tone, intracellular Ca(2+) and myogenic vasoconstriction in resistance arteries (PubMed:18583713). Also involved in unfolded protein response (UPR) and ER stress-induced autophagy via regulation of intracellular S1P levels (PubMed:20798685, PubMed:18583713). Involved in the regulation of epidermal homeostasis and keratinocyte differentiation (By similarity).

亚细胞位置:

Endoplasmic reticulum membrane Multi-pass membrane protein Cell membrane Multi-pass membrane protein

数据库链接

UniGene:

Hs.24678

STRING:

9606.ENSP00000247225

KEGG:

hsa:81537

MIM:

612826

Pfam:

PF01569

Uniprot:

Q9BX95

相关产品

Mouse Sgpp1 ELISA Kit

序列长度:

441

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

448:

FNIPCDDIRK | ARQHMEVELP | YRYITY GMV | GFSITFFVPY | IFFFIGIS

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Role of human sphingosine-1-phosphate phosphatase 1 in the regulation of intra- and extracellular sphingosine-1-phosphate levels and cell viability."

(PMID: 12815058)

[2].

"Sphingosine-1-phosphate phosphohydrolase-1 regulates ER stress-induced autophagy."

(PMID: 20798685)

[3].

"Genetic determinants of circulating sphingolipid concentrations in European populations."

(PMID: 19798445)

[4].

"Role of sphingosine-1-phosphate phosphohydrolase 1 in the regulation of resistance artery tone."

(PMID: 18583713)

[5].

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."

(PMID: 17081983)

[6].

"Role of sphingosine-1-phosphate phosphatase 1 in epidermal growth factor-induced chemotaxis."

(PMID: 15180992)

[7].

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

(PMID: 15489334)

[8].

"Identification and characterization of a novel human sphingosine-1-phosphate phosphohydrolase, hSPP2."

(PMID: 12411432)

[9].

"The DNA sequence and analysis of human chromosome 14."

(PMID: 12508121)

[10].

"Characterization of murine sphingosine-1-phosphate phosphohydrolase."

(PMID: 11756451)

[1].

人磷酸鞘氨醇磷酸酶1(SGPP1)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人磷酸鞘氨醇磷酸酶1(SGPP1)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人磷酸鞘氨醇磷酸酶1(SGPP1)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人磷酸鞘氨醇磷酸酶1(SGPP1)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人磷酸鞘氨醇磷酸酶1(SGPP1)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人磷酸鞘氨醇磷酸酶1(SGPP1)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人磷酸鞘氨醇磷酸酶1(SGPP1)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人磷酸鞘氨醇磷酸酶1(SGPP1)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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