首页 > 重组蛋白 > Human CXCL12 Recombinant Protein

CXCL12 (基因名), Stromal cell-derived factor 1 (蛋白名), sdf1_human.

产品名称:

Human CXCL12/ Stromal cell-derived factor 1 Recombinant Protein
基质细胞衍生因子1

货号:

R0477h

商标:

EIAab^®

监管等级:

RUO

别名:

C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, IRH, PBSF, SDF-1, SDF1, SDF1A, SDF1B

序列号:

P48061

来源:

E.coli

种属:

Human

标签:

His

序列:

22-93aa

预估分子量:

7.92 kDa (monomer)

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Immunology

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 1 )
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 10q11.21 by HGNC 10q11.21 by Entrez Gene 10q11.21 by Ensembl

CXCL12 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Monomer or homodimer; in equilibrium. Dimer formation is induced by non acidic pH and the presence of multivalent anions, and by binding to CXCR4 or heparin. Monomeric form is required for full chemotactic activity and resistance to ischemia/reperfusion injury, whereas the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization but with no chemotactic activity and instead acts as a selective antagonist that blocks chemotaxis induced by the monomeric form. Interacts with the N-terminus of ACKR3.

功能:

Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.

亚细胞位置:

Secreted

数据库链接

UniGene:

Hs.522891

SMR:

P48061

STRING:

9606.ENSP00000379140

KEGG:

hsa:6387

MIM:

600835

Pfam:

PF00048

Uniprot:

P48061

相关产品

Mouse Cxcl12 ELISA Kit

Mouse Cxcl12 CLIA Kit

Mouse Cxcl12 Protein

Mouse Cxcl12 Polyclonal Antibody

Mouse Cxcl12 Monoclonal Antibody

序列长度:

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

93:

NTPNCALQIV | ARLKNNNRQV | CIDPKLKWIQ | EYLEKALNKR | FKM

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"The serum concentrations of chemokine CXCL12 and its specific receptor CXCR4 in patients with esophageal cancer"

M Łukaszewicz-Zając, B Mroczko, M Kozłowski, et al.

From: Disease markers

[1].

"Structure and chromosomal localization of the human stromal cell-derived factor 1 (SDF1) gene."

(PMID: 7490086)

[2].

"HIV infection and host genetic mutation among injecting drug-users of northeastern states of India."

(PMID: 20411675)

[3].

"CXCL12 rs1801157 polymorphism in patients with breast cancer, Hodgkin's lymphoma, and non-Hodgkin's lymphoma."

(PMID: 19927352)

[4].

"Analysis of CCR5, CCR2, SDF1 and RANTES gene polymorphisms in subjects with HIV-related PML and not determined leukoencephalopathy."

(PMID: 17560067)

[5].

"Distribution of HIV-1 resistant polymorphisms among HIV infected patients in Georgia."

(PMID: 19124913)

[1].

人基质细胞衍生因子1(CXCL12)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人基质细胞衍生因子1(CXCL12)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人基质细胞衍生因子1(CXCL12)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人基质细胞衍生因子1(CXCL12)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人基质细胞衍生因子1(CXCL12)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人基质细胞衍生因子1(CXCL12)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人基质细胞衍生因子1(CXCL12)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人基质细胞衍生因子1(CXCL12)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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