首页 > 重组蛋白 > Human PAK1 Recombinant Protein

PAK1 (基因名), Serine/threonine-protein kinase PAK 1 (蛋白名), pak1_human1.

产品名称:

Human PAK1/ Serine/threonine-protein kinase PAK 1 Recombinant Protein
丝氨酸/苏氨酸蛋白激酶1

货号:

R13344h

商标:

EIAab^®

监管等级:

RUO

别名:

Alpha-PAK, p21-activated kinase 1, p65-PAK, PAK-1

序列号:

Q13153

来源:

E.coli

种属:

Human

标签:

His

序列:

1-200aa

预估分子量:

22 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

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Human PAK1 Protein

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产品说明书

说明书:

MSDS:

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数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human PAK1 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 11q13.5-q14.1 by HGNC 11q13.5-q14.1 by Entrez Gene 11q13.5-q14.1 by Ensembl

PAK1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Homodimer in its autoinhibited state. Active as monomer. Component of cytoplasmic complexes, which also contains PXN, ARHGEF6 and GIT1. Interacts with NISCH (By similarity). Interacts with DVL1; mediates the formation of a DVL1, MUSK and PAK1 ternary complex involved in AChR clustering (By similarity). Binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. Interacts with ARHGEF7. Interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. Probably found in a ternary complex composed of DSCAM, PAK1 and RAC1. Interacts with DSCAM (via cytoplasmic domain); the interaction is direct and enhanced in presence of RAC1. Interacts with SCRIB. Interacts with PDPK1. Interacts (via kinase domain) with RAF1. Interacts with NCK1 and NCK2. Interacts with TBCB. Interacts with CRIPAK. Interacts with BRSK2. Interacts with SNAI1. Interacts with CIB1 isoform 2. Interacts with CIB1 (via N-terminal region); the interaction is direct, promotes PAK1 activity and occurs in a calcium-dependent manner.

功能:

Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at 'Ser-246' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation. Plays a role in RUFY3-mediated facilitating gastric cancer cells migration and invasion (PubMed:25766321).

亚细胞位置:

Cytoplasm Cell junction Focal adhesion Cell membrane Cell projection Ruffle membrane Cell projection Invadopodium Colocalizes with RUFY3, F-actin and other core migration components in invadopodia at the cell periphery (PubMed:25766321). Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation. Colocalized with CIB1 within membrane ruffles during cell spreading upon readhesion to fibronectin.

数据库链接

UniGene:

Hs.435714

SMR:

Q13153

STRING:

9606.ENSP00000278568

KEGG:

hsa:5058

MIM:

602590

Pfam:

PF00786

Uniprot:

Q13153

相关产品

Mouse Pak1 ELISA Kit

Rat Pak1 ELISA Kit

Bovine PAK1 ELISA Kit

Mouse Pak1 CLIA Kit

Rat Pak1 CLIA Kit

序列长度:

545

序列:

仅供参考，详细请咨询（services@eiaab.com）

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545:

CLEMDVEKRG | SAKELLQHQF | LKIAKPLSSL | TPLIAAAKEA | TKNNH

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"CRIPak, a novel endogenous Pak1 inhibitor."

(PMID: 16278681)

[2].

"Integrin engagement differentially modulates epithelial cell motility by RhoA/ROCK and PAK1."

(PMID: 15611088)

[3].

"Pak1 phosphorylation of snail, a master regulator of epithelial-to-mesenchyme transition, modulates snail's subcellular localization and functions."

(PMID: 15833848)

[4].

"The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis."

(PMID: 12624090)

[5].

"Pak1 kinase homodimers are autoinhibited in trans and dissociated upon activation by Cdc42 and Rac1."

(PMID: 11804587)

[6].

"Identification of a central phosphorylation site in p21-activated kinase regulating autoinhibition and kinase activity."

(PMID: 10551809)

[7].

"Differential effects of PAK1-activating mutations reveal activity-dependent and -independent effects on cytoskeletal regulation."

(PMID: 9774440)

[8].

"Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells."

(PMID: 9395435)

[9].

"Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway."

(PMID: 8805275)

[10].

"Glucose-regulated protein 78 (GRP78) binds directly to PIP3 phosphatase SKIP and determines its localization."

(PMID: 26940976)

[1].

人丝氨酸/苏氨酸蛋白激酶1(PAK1)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人丝氨酸/苏氨酸蛋白激酶1(PAK1)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人丝氨酸/苏氨酸蛋白激酶1(PAK1)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人丝氨酸/苏氨酸蛋白激酶1(PAK1)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人丝氨酸/苏氨酸蛋白激酶1(PAK1)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人丝氨酸/苏氨酸蛋白激酶1(PAK1)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人丝氨酸/苏氨酸蛋白激酶1(PAK1)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人丝氨酸/苏氨酸蛋白激酶1(PAK1)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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