首页 > 重组蛋白 > Human P4HA1 Recombinant Protein

P4HA1 (基因名), Prolyl 4-hydroxylase subunit alpha-1 (蛋白名), p4ha1_human.

产品名称:

Human P4HA1/ Prolyl 4-hydroxylase subunit alpha-1 Recombinant Protein
脯氨酰羟化酶

货号:

R10871h

商标:

EIAab^®

监管等级:

RUO

别名:

Procollagen-proline, 2-oxoglutarate-4-dioxygenase subunit alpha-1, 4-PH alpha-1, P4HA

序列号:

P13674

来源:

E.coli

种属:

Human

标签:

His

序列:

236-336aa

预估分子量:

11.11 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

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Human P4HA1 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

The sequence analysis of human P4HA1 protein including hydrophilcity, hydrophobicity and antigenicity.

Recombinant human P4HA1 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 10q22.1 by HGNC 10q22.1 by Entrez Gene 10q22.1 by Ensembl

P4HA1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Heterotetramer of two alpha-1 chains and two beta chains (the beta chain is the multi-functional PDI).

功能:

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

亚细胞位置:

Endoplasmic reticulum lumen

数据库链接

UniGene:

Hs.500047

SMR:

P13674

STRING:

9606.ENSP00000263556

KEGG:

hsa:5033

MIM:

176710

Pfam:

PF13640

Uniprot:

P13674

相关产品

Mouse P4ha1 ELISA Kit

Rat P4ha1 ELISA Kit

Bovine P4HA1 ELISA Kit

Chicken P4HA1 ELISA Kit

Mouse P4ha1 CLIA Kit

序列长度:

534

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

534:

AACPVLVGNK | WVSNKWLHER | GQEFRRPCTL | SELE

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score."

(PMID: 20379614)

[2].

"A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease."

(PMID: 16385451)

[3].

"PCR isolation and cloning of novel splice variant mRNAs from known drug target genes."

(PMID: 15028279)

[4].

"The DNA sequence and comparative analysis of human chromosome 10."

(PMID: 15164054)

[5].

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

(PMID: 15489334)

[6].

"The order and transcriptional orientation of the human COL13A1 and P4HA genes on chromosome 10 long arm determined by high-resolution FISH."

(PMID: 9417920)

[7].

"Structure and expression of the human gene for the alpha subunit of prolyl 4-hydroxylase. The two alternatively spliced types of mRNA correspond to two homologous exons the sequences of which are expressed in a variety of tissues."

(PMID: 7961714)

[8].

"Assignment of the gene coding for the alpha-subunit of prolyl 4-hydroxylase to human chromosome region 10q21.3-23.1."

(PMID: 2556027)

[9].

"Molecular cloning of the alpha-subunit of human prolyl 4-hydroxylase: the complete cDNA-derived amino acid sequence and evidence for alternative splicing of RNA transcripts."

(PMID: 2543975)

[10].

"Architecture of the human interactome defines protein communities and disease networks."

(PMID: 28514442)

[1].

人脯氨酰羟化酶(P4HA1)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人脯氨酰羟化酶(P4HA1)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人脯氨酰羟化酶(P4HA1)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人脯氨酰羟化酶(P4HA1)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人脯氨酰羟化酶(P4HA1)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人脯氨酰羟化酶(P4HA1)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人脯氨酰羟化酶(P4HA1)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人脯氨酰羟化酶(P4HA1)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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