首页 > 重组蛋白 > Human NCKAP1L Recombinant Protein

NCKAP1L (基因名), Nck-associated protein 1-like (蛋白名), nckpl_human.

产品名称:

Human NCKAP1L/ Nck-associated protein 1-like Recombinant Protein

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

Hematopoietic protein 1, Membrane-associated protein HEM-1, HEM1

序列号:

P55160

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

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Human NCKAP1L Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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基因位点

Cytogenetic band: 12q13.13-q13.2 by HGNC 12q13.13-q13.2 by Entrez Gene 12q13.13-q13.2 by Ensembl

NCKAP1L Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

In hematopoietic cells, component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1L/HEM1 and WASF2/WAVE2 (PubMed:16417406). Interacts with ARHGAP4, PIK3C3/VPS34 and PPP1R12A/MYPT1 (PubMed:16417406).

功能:

Essential hematopoietic-specific regulator of the actin cytoskeleton (Probable). Controls lymphocyte development, activation, proliferation and homeostasis, erythrocyte membrane stability, as well as phagocytosis and migration by neutrophils and macrophages (PubMed:16417406, PubMed:17696648). Component of the WAVE2 complex which signals downstream of RAC to stimulate F-actin polymerization. Required for stabilization and/or translation of the WAVE2 complex proteins in hematopoietic cells (By similarity). Exhibits complex cycles of activation and inhibition to generate waves of propagating the assembly with actin (PubMed:16417406). Also involved in mechanisms WAVE-independent to regulate myosin and actin polymerization during neutrophil chemotaxis (PubMed:17696648).

亚细胞位置:

Cell membrane Single-pass membrane protein Cytoplasmic side Cytoplasm Localizes to the leading edge of polarized neutrophils.

数据库链接

UniGene:

Hs.182014

SMR:

P55160

STRING:

9606.ENSP00000293373

KEGG:

hsa:3071

MIM:

141180

Pfam:

PF09735

Uniprot:

P55160

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序列长度:

1127

序列:

仅供参考，详细请咨询（services@eiaab.com）

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1127:

LDMLESCFPY | VLLRNAYREV | SRAFHLN

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis."

(PMID: 16417406)

[2].

"Hem-1, a potential membrane protein, with expression restricted to blood cells."

(PMID: 1932118)

[3].

"An actin-based wave generator organizes cell motility."

(PMID: 17696648)

[4].

"The HEM proteins: a novel family of tissue-specific transmembrane proteins expressed from invertebrates through mammals with an essential function in oogenesis."

(PMID: 7643388)

[5].

"Architecture of the human interactome defines protein communities and disease networks."

(PMID: 28514442)

[6].

"Panorama of ancient metazoan macromolecular complexes."

(PMID: 26344197)

[7].

"Cell Surface Proteomic Map of HIV Infection Reveals Antagonism of Amino Acid Metabolism by Vpu and Nef."

(PMID: 26439863)

[8].

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."

(PMID: 24275569)

[9].

"NOTCH1 nuclear interactome reveals key regulators of its transcriptional activity and oncogenic function."

(PMID: 23022380)

[10].

"Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population."

(PMID: 23251661)

[1].

人NCKAP1L重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人NCKAP1L重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人NCKAP1L重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人NCKAP1L重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人NCKAP1L重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人NCKAP1L重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人NCKAP1L重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人NCKAP1L重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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