首页 > 重组蛋白 > Human MELK Recombinant Protein

MELK (基因名), Maternal embryonic leucine zipper kinase (蛋白名), melk_human.

产品名称:

Human MELK/ Maternal embryonic leucine zipper kinase Recombinant Protein
母体胚胎亮氨酸拉链激

货号:

R15058h

商标:

EIAab^®

监管等级:

RUO

别名:

Protein kinase Eg3, Protein kinase PK38, Tyrosine-protein kinase MELK, pEg3 kinase, hPK38, hMELK, KIAA0175

序列号:

Q14680

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

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Human MELK Protein

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产品说明书

说明书:

MSDS:

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数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 9p13.2 by HGNC 9p13.2 by Entrez Gene 9p13.2 by Ensembl

MELK Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Monomer. Interacts with ZNF622 and PPP1R8.

功能:

Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.

亚细胞位置:

Cell membrane Peripheral membrane protein

数据库链接

UniGene:

Hs.184339

SMR:

Q14680

STRING:

9606.ENSP00000298048

KEGG:

hsa:9833

MIM:

607025

Pfam:

PF02149

Uniprot:

Q14680

相关产品

Mouse Melk ELISA Kit

Mouse Melk CLIA Kit

Mouse Melk Protein

Mouse Melk Polyclonal Antibody

Mouse Melk Monoclonal Antibody

序列长度:

651

序列:

仅供参考，详细请咨询（services@eiaab.com）

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3D 模型:

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免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Dysregulated expression of Fau and MELK is associated with poor prognosis in breast cancer."

(PMID: 19671159)

[2].

"Maternal embryonic leucine zipper kinase is a key regulator of the proliferation of malignant brain tumors, including brain tumor stem cells."

(PMID: 17722061)

[3].

"Maternal embryonic leucine zipper kinase transcript abundance correlates with malignancy grade in human astrocytomas."

(PMID: 17960622)

[4].

"Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family."

(PMID: 17280616)

[5].

"CDC25B phosphorylated by pEg3 localizes to the centrosome and the spindle poles at mitosis."

(PMID: 15908796)

[6].

"Substrate specificity and activity regulation of protein kinase MELK."

(PMID: 16216881)

[7].

"Inhibition of spliceosome assembly by the cell cycle-regulated protein kinase MELK and involvement of splicing factor NIPP1."

(PMID: 14699119)

[8].

"Human pEg3 kinase associates with and phosphorylates CDC25B phosphatase: a potential role for pEg3 in cell cycle regulation."

(PMID: 12400006)

[9].

"Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1."

(PMID: 8724849)

[10].

"Siomycin A targets brain tumor stem cells partially through a MELK-mediated pathway."

(PMID: 21558073)

[1].

人母体胚胎亮氨酸拉链激(MELK)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人母体胚胎亮氨酸拉链激(MELK)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人母体胚胎亮氨酸拉链激(MELK)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人母体胚胎亮氨酸拉链激(MELK)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人母体胚胎亮氨酸拉链激(MELK)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人母体胚胎亮氨酸拉链激(MELK)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人母体胚胎亮氨酸拉链激(MELK)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人母体胚胎亮氨酸拉链激(MELK)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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