首页 > 重组蛋白 > Human ABCB4 Recombinant Protein

ABCB4 (基因名), Phosphatidylcholine translocator ABCB4 (蛋白名), mdr3_human.

产品名称:

Human ABCB4/ Phosphatidylcholine translocator ABCB4 Recombinant Protein
多药耐药蛋白3

货号:

R10409h

商标:

EIAab^®

监管等级:

RUO

别名:

ATP-binding cassette sub-family B member 4, Multidrug resistance protein 3, P-glycoprotein 3, MDR3, PGY3

序列号:

P21439

来源:

E.coli

种属:

Human

标签:

His

序列:

Recombinant protein

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cardiovascular

规格 & 价格:

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Human ABCB4 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 7q21.12 by HGNC 7q21.12 by Entrez Gene 7q21.12 by Ensembl

ABCB4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

May interact with RACK1 (PubMed:19674157). Interacts with HAX1.

功能:

Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi of hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts (PubMed:7957936, PubMed:8898203, PubMed:9366571, PubMed:17523162, PubMed:23468132, PubMed:24806754, PubMed:24723470, PubMed:24594635, PubMed:21820390). Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to fu rther enrichment of SM and cholesterol in raft membranes in hepatocytes (PubMed:23468132). Required for proper phospholipid bile formation (By similarity). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner (PubMed:24045840). May promote biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane (PubMed:9366571). In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts (PubMed:21820390). Does not confer multidrug resistance.

亚细胞位置:

Cell membrane Multi-pass membrane protein Apical cell membrane Multi-pass membrane protein Membrane raft Cytoplasm Cytoplasmic vesicle Clathrin-coated vesicle Localized at the apical canalicular membrane of the epithelial cells lining the lumen of the bile canaliculi and biliary ductules (By similarity). Transported from the Golgi to the apical bile canalicular membrane in a RACK1-dependent manner (PubMed:19674157). Redistributed into pseudocanaliculi formed between cells in a bezafibrate- or PPARA-dependent manner (PubMed:15258199). Localized preferentially in lipid nonraft domains of canalicular plasma membranes (PubMed:23468132).

数据库链接

UniGene:

Hs.654403

SMR:

P21439

STRING:

9606.ENSP00000265723

KEGG:

hsa:5244

MIM:

171060

Pfam:

PF00664

Uniprot:

P21439

相关产品

Mouse Abcb4 ELISA Kit

序列长度:

1286

序列:

仅供参考，详细请咨询（services@eiaab.com）

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1286:

VFQNGRVKEH | GTHQQLLAQK | GIYFSMVSVQ | AGTQNL

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"A new splicing site mutation of the ABCB4 gene in intrahepatic cholestasis of pregnancy with raised serum gamma-GT."

(PMID: 19261551)

[2].

"Mutations and polymorphisms in the bile salt export pump and the multidrug resistance protein 3 associated with drug-induced liver injury."

(PMID: 17264802)

[3].

"Genetic variability, haplotype structures, and ethnic diversity of hepatic transporters MDR3 (ABCB4) and bile salt export pump (ABCB11)."

(PMID: 16763017)

[4].

"Sequence analysis of bile salt export pump (ABCB11) and multidrug resistance p-glycoprotein 3 (ABCB4, MDR3) in patients with intrahepatic cholestasis of pregnancy."

(PMID: 15077010)

[5].

"MDR3 gene defect in adults with symptomatic intrahepatic and gallbladder cholesterol cholelithiasis."

(PMID: 11313316)

[6].

"Common variants of ABCB4 and ABCB11 and plasma lipid levels: a study in sib pairs with gallstones, and controls."

(PMID: 19408031)

[7].

"Receptor for activated C-kinase 1 regulates the cellular localization and function of ABCB4."

(PMID: 19674157)

[8].

"ABCB4 gene mutations and single-nucleotide polymorphisms in women with intrahepatic cholestasis of pregnancy."

(PMID: 19584064)

[9].

"ABCB4 sequence variations in young adults with cholesterol gallstone disease."

(PMID: 19018976)

[10].

"ABCB4 heterozygous gene mutations associated with fibrosing cholestatic liver disease in adults."

(PMID: 18482588)

[1].

人多药耐药蛋白3(ABCB4)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人多药耐药蛋白3(ABCB4)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人多药耐药蛋白3(ABCB4)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人多药耐药蛋白3(ABCB4)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人多药耐药蛋白3(ABCB4)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人多药耐药蛋白3(ABCB4)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人多药耐药蛋白3(ABCB4)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人多药耐药蛋白3(ABCB4)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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