首页 > 重组蛋白 > Human JAK3 Recombinant Protein

JAK3 (基因名), Tyrosine-protein kinase JAK3 (蛋白名), jak3_human.

产品名称:

Human JAK3/ Tyrosine-protein kinase JAK3 Recombinant Protein
酪氨酸蛋白激酶JAK3

货号:

R0425h

商标:

EIAab^®

监管等级:

RUO

别名:

Janus kinase 3, Leukocyte janus kinase, JAK-3, L-JAK

序列号:

P52333

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Signal Transduction

规格 & 价格:

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Human JAK3 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 19p13.11 by HGNC 19p13.11 by Entrez Gene 19p13.11 by Ensembl

JAK3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with STAM2 and MYO18A (By similarity). Interacts with SHB.

功能:

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.

亚细胞位置:

Endomembrane system Peripheral membrane protein Cytoplasm

数据库链接

UniGene:

Hs.515247

SMR:

P52333

STRING:

9606.ENSP00000391676

KEGG:

hsa:3718

MIM:

600173

Pfam:

PF07714

Uniprot:

P52333

相关产品

序列长度:

1124

序列:

仅供参考，详细请咨询（services@eiaab.com）

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1124:

GSRGCETHAF | TAHPEGKHHS | LSFS

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Genomic sequence, organization, and chromosomal localization of human JAK3."

(PMID: 8921370)

[2].

"Association of single-nucleotide polymorphisms in JAK3, STAT4, and STAT6 with new cardiovascular events in incident dialysis patients."

(PMID: 19282076)

[3].

"Phosphorylation of human Jak3 at tyrosines 904 and 939 positively regulates its activity."

(PMID: 18250158)

[4].

"Janus kinase 3 (JAK3) deficiency: clinical, immunologic, and molecular analyses of 10 patients and outcomes of stem cell transplantation."

(PMID: 14615376)

[5].

"The pseudokinase domain is required for suppression of basal activity of Jak2 and Jak3 tyrosine kinases and for cytokine-inducible activation of signal transduction."

(PMID: 12351625)

[6].

"Structural and functional basis for JAK3-deficient severe combined immunodeficiency."

(PMID: 9354668)

[7].

"Expression of Janus kinase 3 in human endothelial and other non-lymphoid and non-myeloid cells."

(PMID: 8662778)

[8].

"Regulation of JAK3 expression in human monocytes: phosphorylation in response to interleukins 2, 4, and 7."

(PMID: 7535338)

[9].

"A kinase-deficient splice variant of the human JAK3 is expressed in hematopoietic and epithelial cancer cells."

(PMID: 7559633)

[10].

"Mutations of Jak-3 gene in patients with autosomal severe combined immune deficiency (SCID)."

(PMID: 7659163)

[1].

人酪氨酸蛋白激酶JAK3(JAK3)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人酪氨酸蛋白激酶JAK3(JAK3)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人酪氨酸蛋白激酶JAK3(JAK3)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人酪氨酸蛋白激酶JAK3(JAK3)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人酪氨酸蛋白激酶JAK3(JAK3)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人酪氨酸蛋白激酶JAK3(JAK3)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人酪氨酸蛋白激酶JAK3(JAK3)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人酪氨酸蛋白激酶JAK3(JAK3)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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