H3F3A (基因名), Histone H3.3 (蛋白名), h33_human.
产品名称:
Human H3F3A/ Histone H3.3 Recombinant Protein
组蛋白H3.3
货号:
R5810h
商标:
EIAab®
监管等级:
别名:
PP781, H3.3A, H3F3
序列号:
P84243
来源:
E.coli
种属:
Human
标签:
His
序列:
1-81aa
预估分子量:
8.91 kDa (monomer)
纯度:
Greater than 98% by SDS-PAGE
浓度:
Reconstitution Dependent
形态:
Liquid
内毒素水平:
Please contact protein@eiaab.com The technician for more information.
应用:
存储缓冲液:
50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
存储:
Store at -20°C. (Avoid repeated freezing and thawing.)
研究领域:
-
R&D 技术数据
The PCR product of human H3F3A gene was determined by 1% Agarose stained with EB.
Recombinant human H3F3A protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.
基因位点
H3F3A Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
通用注释
亚单元:
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes. Interacts with ZMYND11; when trimethylated at 'Lys-36' (H3.3K36me3).
功能:
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
亚细胞位置:
Nucleus
Chromosome
数据库链接
验证序列:
该产品尚未在任何出版物中被引用。
[2].
"Unusual structure, evolutionary conservation of non-coding sequences and numerous pseudogenes characterize the human H3.3 histone multigene family."
[4].
"ZMYND11 links histone H3.3K36me3 to transcription elongation and tumour suppression."
[5].
"Distinct H3F3A and H3F3B driver mutations define chondroblastoma and giant cell tumor of bone."
[6].
"The histone H3.3K27M mutation in pediatric glioma reprograms H3K27 methylation and gene expression."
[7].
"Inhibition of PRC2 activity by a gain-of-function H3 mutation found in pediatric glioblastoma."
[8].
"Driver mutations in histone H3.3 and chromatin remodelling genes in paediatric glioblastoma."
[9].
"Organismal differences in post-translational modifications in histones H3 and H4."
[10].
"Identification of histone H3 lysine 36 acetylation as a highly conserved histone modification."
[1].
人组蛋白H3.3(H3F3A)重组蛋白是否是无菌的？
蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。
[2].
人组蛋白H3.3(H3F3A)重组蛋白的保存缓冲液是什么？
纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。
[3].
怎样确定人组蛋白H3.3(H3F3A)重组蛋白的浓度？
蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。
[4].
人组蛋白H3.3(H3F3A)重组蛋白蛋白保存条件是怎样的？
蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。
[5].
人组蛋白H3.3(H3F3A)重组蛋白是否可以用于活体实验？
重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。
[6].
人组蛋白H3.3(H3F3A)重组蛋白的保质期是多久？
在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。
[7].
你们蛋白和抗体的报价是怎么样的？
我们将根据你需要的蛋白和抗体的大小进行报价。
[8].
人组蛋白H3.3(H3F3A)重组蛋白是否能够提供蛋白片段？
我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。
[9].
人组蛋白H3.3(H3F3A)重组蛋白的货期或发货时间一般是多长？
具体指标的货期需要确定。最快一周，最长可能一个月。
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