首页 > 重组蛋白 > Human CHD8 Recombinant Protein

CHD8 (基因名), Chromodomain-helicase-DNA-binding protein 8 (蛋白名), chd8_human1.

产品名称:

Human CHD8/ Chromodomain-helicase-DNA-binding protein 8 Recombinant Protein
Chromodomain-helicase-DNA-binding蛋白质8

货号:

R11393h

商标:

EIAab^®

监管等级:

RUO

别名:

ATP-dependent helicase CHD8, Helicase with SNF2 domain 1, CHD-8, HELSNF1, KIAA1564

序列号:

Q9HCK8

来源:

E.coli

种属:

Human

标签:

His

序列:

570-720aa

预估分子量:

16.61 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

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Human CHD8 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human CHD8 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 14q11.2 by HGNC 14q11.2 by Entrez Gene 14q11.2 by Ensembl

CHD8 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with p53/TP53, histone H1, CTNNB1, CTCF and PIAS3. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with CHD7. Interacts with FAM124B (PubMed:23285124).

功能:

DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription.

亚细胞位置:

Nucleus Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites.

数据库链接

UniGene:

Hs.530698

SMR:

Q9HCK8

STRING:

9606.ENSP00000382863

KEGG:

hsa:57680

MIM:

610528

Pfam:

PF07533

Uniprot:

Q9HCK8

相关产品

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SQGYDSSERD | FSLIDDPMMP | ANSDSSEDAD | D

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription."

(PMID: 17938208)

[2].

"Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."

(PMID: 10997877)

[3].

"Multiplex targeted sequencing identifies recurrently mutated genes in autism spectrum disorders."

(PMID: 23160955)

[4].

"CHD8 interacts with CHD7, a protein which is mutated in CHARGE syndrome."

(PMID: 20453063)

[5].

"Regulation of androgen-responsive transcription by the chromatin remodeling factor CHD8."

(PMID: 20308527)

[6].

"Regulation of HOXA2 gene expression by the ATP-dependent chromatin remodeling enzyme CHD8."

(PMID: 20085832)

[7].

"The chromatin remodeling factor CHD8 interacts with elongating RNA polymerase II and controls expression of the cyclin E2 gene."

(PMID: 19255092)

[8].

"CHD8 is an ATP-dependent chromatin remodeling factor that regulates beta-catenin target genes."

(PMID: 18378692)

[9].

"CTCF-dependent chromatin insulator is linked to epigenetic remodeling."

(PMID: 16949368)

[10].

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."

(PMID: 16964243)

[1].

人Chromodomain-helicase-DNA-binding蛋白质8(CHD8)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人Chromodomain-helicase-DNA-binding蛋白质8(CHD8)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人Chromodomain-helicase-DNA-binding蛋白质8(CHD8)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人Chromodomain-helicase-DNA-binding蛋白质8(CHD8)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人Chromodomain-helicase-DNA-binding蛋白质8(CHD8)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人Chromodomain-helicase-DNA-binding蛋白质8(CHD8)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人Chromodomain-helicase-DNA-binding蛋白质8(CHD8)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人Chromodomain-helicase-DNA-binding蛋白质8(CHD8)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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