首页 > 重组蛋白 > Human CCN1 Recombinant Protein

CCN1 (基因名), CCN family member 1 (蛋白名), ccn1_human.

产品名称:

Human CCN1/ CCN family member 1 Recombinant Protein
CYR61蛋白

货号:

R0494h

商标:

EIAab^®

监管等级:

RUO

别名:

Cellular communication network factor 1, Cysteine-rich angiogenic inducer 61, Insulin-like growth factor-binding protein 10, Protein CYR61, Protein GIG1, IBP-10, CYR61, GIG1, IGFBP10

序列号:

O00622

来源:

E.coli

种属:

Human

标签:

His

序列:

171-381aa

预估分子量:

23.21 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cardiovascular

规格 & 价格:

关注

Human CCN1 Protein

邮箱 *

消息 *

Please 登录.

产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
反馈墙 (0)

R&D 技术数据

Recombinant human CCN1 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 1p22.3 by HGNC 1p22.3 by Entrez Gene 1p22.3 by Ensembl

CCN1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interaction with integrins is heparin- and cell-type-dependent and promotes cell adhesion. In skin fibroblasts it binds ITGA6/ITGB1, in endothelial cells, binds ITGAV/ITGB3 and in platelets, ITGA2B/ITGB3. Binds, in vitro, ITGAV/ITGB5.

功能:

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.

亚细胞位置:

Secreted

数据库链接

UniGene:

Hs.8867

SMR:

O00622

STRING:

9606.ENSP00000398736

KEGG:

hsa:3491

MIM:

602369

Pfam:

PF00007

Uniprot:

O00622

相关产品

Mouse Ccn1 ELISA Kit

Rat Ccn1 ELISA Kit

Chicken CCN1 ELISA Kit

Mouse Ccn1 CLIA Kit

Rat Ccn1 CLIA Kit

序列长度:

381

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

387:

NVMMIQSCKC | NYNCP HANE | AAFPFYRLFN | DIHKFRD

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"The human growth factor-inducible immediate early gene, CYR61, maps to chromosome 1p."

(PMID: 9135077)

[2].

"Organization and expression of the Cyr61 gene in normal human fibroblasts."

(PMID: 11810026)

[3].

"The angiogenic factor Cyr61 activates a genetic program for wound healing in human skin fibroblasts."

(PMID: 11584015)

[4].

"CYR61 stimulates human skin fibroblast migration through Integrin alpha vbeta 5 and enhances mitogenesis through integrin alpha vbeta 3, independent of its carboxyl-terminal domain."

(PMID: 11287419)

[5].

"Muscarinic acetylcholine receptors induce the expression of the immediate early growth regulatory gene CYR61."

(PMID: 10852911)

[6].

"Adhesion of human skin fibroblasts to Cyr61 is mediated through integrin alpha 6beta 1 and cell surface heparan sulfate proteoglycans."

(PMID: 10821835)

[7].

"Activation-dependent adhesion of human platelets to Cyr61 and Fisp12/mouse connective tissue growth factor is mediated through integrin alpha(IIb)beta(3)."

(PMID: 10446209)

[8].

"Adhesion of human umbilical vein endothelial cells to the immediate-early gene product Cyr61 is mediated through integrin alphavbeta3."

(PMID: 9446626)

[9].

"Chromosomal mapping and expression of the human cyr61 gene in tumour cells from the nervous system."

(PMID: 9536281)

[10].

"Regulation of the matricellular proteins CYR61 (CCN1) and NOV (CCN3) by hypoxia-inducible factor-1{alpha} and transforming-growth factor-{beta}3 in the human trophoblast."

(PMID: 20237132)

[1].

人CYR61蛋白(CCN1)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人CYR61蛋白(CCN1)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人CYR61蛋白(CCN1)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人CYR61蛋白(CCN1)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人CYR61蛋白(CCN1)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人CYR61蛋白(CCN1)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人CYR61蛋白(CCN1)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人CYR61蛋白(CCN1)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

反馈墙

评论数 : 0

所有用户