CCL5 (基因名), C-C motif chemokine 5 (蛋白名), ccl5_human.
产品名称:
Human CCL5/ C-C motif chemokine 5 Recombinant Protein
C-C模体趋化因子5
货号:
R0116h
商标:
EIAab®
监管等级:
别名:
EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, TCP228, D17S136E, SCYA5
序列号:
P13501
来源:
E.coli
种属:
Human
标签:
His
序列:
24-91aa
预估分子量:
7.48 kDa (monomer)
纯度:
Greater than 95% by SDS-PAGE
浓度:
Reconstitution Dependent
形态:
Liquid
内毒素水平:
Please contact protein@eiaab.com The technician for more information.
应用:
存储缓冲液:
50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
存储:
Store at -20°C. (Avoid repeated freezing and thawing.)
研究领域:
Neurosciences
R&D 技术数据
The sequence analysis of human CCL5 protein including hydrophilcity, hydrophobicity and antigenicity.
Recombinant human CCL5 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.
基因位点
CCL5 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
通用注释
亚单元:
N/A
功能:
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils (PubMed:16791620, PubMed:1380064, PubMed:8525373, PubMed:9516414, PubMed:15923218). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells (PubMed:23979485).
亚细胞位置:
Secreted
数据库链接
验证序列:
该产品尚未在任何出版物中被引用。
[2].
"Analysis of CCR5, CCR2, SDF1 and RANTES gene polymorphisms in subjects with HIV-related PML and not determined leukoencephalopathy."
[3].
"RANTES stimulates Ca2+ mobilization and inositol trisphosphate (IP3) formation in cells transfected with G protein-coupled receptor 75."
[4].
"Behavioral risk exposure and host genetics of susceptibility to HIV-1 infection."
[5].
"RANTES gene single nucleotide polymorphisms and expression in patients with chronic hepatitis B virus infection."
[6].
"The CCR5-delta32 mutation: impact on disease outcome in individuals with hepatitis C infection from a single source."
[7].
"Cytokine and chemokine gene polymorphisms among ethnically diverse North Americans with HIV-1 infection."
[8].
"Haplotype-tagging RANTES gene variants influence response to antiviral therapy in chronic hepatitis C."
[9].
"Proton NMR assignments and solution conformation of RANTES, a chemokine of the C-C type."
[10].
"The novel chemokine receptor, G-protein-coupled receptor 75, is expressed by islets and is coupled to stimulation of insulin secretion and improved glucose homeostasis."
[1].
人C-C模体趋化因子5(CCL5)重组蛋白是否是无菌的？
蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。
[2].
人C-C模体趋化因子5(CCL5)重组蛋白的保存缓冲液是什么？
纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。
[3].
怎样确定人C-C模体趋化因子5(CCL5)重组蛋白的浓度？
蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。
[4].
人C-C模体趋化因子5(CCL5)重组蛋白蛋白保存条件是怎样的？
蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。
[5].
人C-C模体趋化因子5(CCL5)重组蛋白是否可以用于活体实验？
重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。
[6].
人C-C模体趋化因子5(CCL5)重组蛋白的保质期是多久？
在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。
[7].
你们蛋白和抗体的报价是怎么样的？
我们将根据你需要的蛋白和抗体的大小进行报价。
[8].
人C-C模体趋化因子5(CCL5)重组蛋白是否能够提供蛋白片段？
我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。
[9].
人C-C模体趋化因子5(CCL5)重组蛋白的货期或发货时间一般是多长？
具体指标的货期需要确定。最快一周，最长可能一个月。
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