首页 > 重组蛋白 > Human BUB1B Recombinant Protein

BUB1B (基因名), Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (蛋白名), bub1b_human.

产品名称:

Human BUB1B/ Mitotic checkpoint serine/threonine-protein kinase BUB1 beta Recombinant Protein

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

MAD3/BUB1-related protein kinase, Mitotic checkpoint kinase MAD3L, Protein SSK1, hBUBR1, BUBR1, MAD3L, SSK1

序列号:

O60566

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

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Human BUB1B Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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基因位点

Cytogenetic band: 15q15.1 by HGNC 15q15.1 by Entrez Gene 15q15.1 by Ensembl

BUB1B Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with KNL1.

功能:

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

亚细胞位置:

Cytoplasm Nucleus Chromosome Centromere Kinetochore Cytoplasm Cytoskeleton Microtubule organizing center Centrosome Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and KNL1.

数据库链接

UniGene:

Hs.513645

SMR:

O60566

STRING:

9606.ENSP00000287598

KEGG:

hsa:701

MIM:

176430

Pfam:

PF08311

Uniprot:

O60566

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序列长度:

1050

序列:

仅供参考，详细请咨询（services@eiaab.com）

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3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner."

(PMID: 9889005)

[2].

"BubR1 acetylation at prometaphase is required for modulating APC/C activity and timing of mitosis."

(PMID: 19407811)

[3].

"BubR1 localizes to centrosomes and suppresses centrosome amplification via regulating Plk1 activity in interphase cells."

(PMID: 19503101)

[4].

"Depletion of BubR1 promotes premature centrosomal localization of cyclin B1 and accelerates mitotic entry."

(PMID: 19411850)

[5].

"Phosphorylation sites in BubR1 that regulate kinetochore attachment, tension, and mitotic exit."

(PMID: 19015317)

[6].

"Genetic variation in the major mitotic checkpoint genes does not affect familial breast cancer risk."

(PMID: 17268814)

[7].

"Polo-like kinase 1 facilitates chromosome alignment during prometaphase through BubR1."

(PMID: 17376779)

[8].

"Human Blinkin/AF15q14 is required for chromosome alignment and the mitotic checkpoint through direct interaction with Bub1 and BubR1."

(PMID: 17981135)

[9].

"Monoallelic BUB1B mutations and defective mitotic-spindle checkpoint in seven families with premature chromatid separation (PCS) syndrome."

(PMID: 16411201)

[10].

"Caspase-mediated specific cleavage of BubR1 is a determinant of mitotic progression."

(PMID: 16227576)

[1].

人BUB1B重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人BUB1B重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人BUB1B重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人BUB1B重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人BUB1B重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人BUB1B重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人BUB1B重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人BUB1B重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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