首页 > 重组蛋白 > Human BTK Recombinant Protein

BTK (基因名), Tyrosine-protein kinase BTK (蛋白名), btk_human.

产品名称:

Human BTK/ Tyrosine-protein kinase BTK Recombinant Protein
BTK酪氨酸蛋白激

货号:

R1915h

商标:

EIAab^®

监管等级:

RUO

别名:

Agammaglobulinemia tyrosine kinase, B-cell progenitor kinase, Bruton tyrosine kinase, ATK, BPK, AGMX1, ATK, BPK

序列号:

Q06187

来源:

E.coli

种属:

Human

标签:

His

序列:

185-299aa

预估分子量:

12.65 kDa (monomer)

纯度:

Greater than 98% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Immunology

规格 & 价格:

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Human BTK Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

The sequence analysis of human BTK protein including hydrophilcity, hydrophobicity and antigenicity.

Recombinant human BTK protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: Xq22.1 by HGNC Xq22.1 by Entrez Gene Xq22.1 by Ensembl

BTK Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Binds GTF2I through the PH domain. Interacts with SH3BP5 via the SH3 domain. Interacts with IBTK via its PH domain. Interacts with ARID3A, CAV1, FASLG, PIN1, TLR8 and TLR9.

功能:

Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.

亚细胞位置:

Cytoplasm Cell membrane Peripheral membrane protein Nucleus In steady state, BTK is predominantly cytosolic. Following B-cell receptor (BCR) engagement by antigen, translocates to the plasma membrane through its PH domain. Plasma membrane localization is a critical step in the activation of BTK. A fraction of BTK also shuttles between the nucleus and the cytoplasm, and nuclear export is mediated by the nuclear export receptor CRM1.

数据库链接

UniGene:

Hs.159494

SMR:

Q06187

STRING:

9606.ENSP00000308176

KEGG:

hsa:695

MIM:

300300

Pfam:

PF00779

Uniprot:

Q06187

相关产品

Mouse Btk ELISA Kit

Chicken BTK ELISA Kit

Mouse Btk CLIA Kit

Chicken BTK CLIA Kit

Mouse Btk Protein

序列长度:

659

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

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450:

500:

550:

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650:

669:

RPTFKILLSN | ILDVMDEES

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Structures of human Bruton's tyrosine kinase in active and inactive conformations suggest a mechanism of activation for TEC family kinases."

(PMID: 20052711)

[2].

"Genotype-phenotype correlation in Bruton's tyrosine kinase deficiency."

(PMID: 18776760)

[3].

"Signaling by Toll-like receptors 8 and 9 requires Bruton's tyrosine kinase."

(PMID: 17932028)

[4].

"Bruton's tyrosine kinase is required for TLR2 and TLR4-induced TNF, but not IL-6, production."

(PMID: 16517732)

[5].

"Functional interaction of caveolin-1 with Bruton's tyrosine kinase and Bmx."

(PMID: 11751885)

[1].

人BTK酪氨酸蛋白激(BTK)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人BTK酪氨酸蛋白激(BTK)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人BTK酪氨酸蛋白激(BTK)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人BTK酪氨酸蛋白激(BTK)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人BTK酪氨酸蛋白激(BTK)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人BTK酪氨酸蛋白激(BTK)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人BTK酪氨酸蛋白激(BTK)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人BTK酪氨酸蛋白激(BTK)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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