首页 > 重组蛋白 > Human BAP1 Recombinant Protein

BAP1 (基因名), Ubiquitin carboxyl-terminal hydrolase BAP1 (蛋白名), bap1_human.

产品名称:

Human BAP1/ Ubiquitin carboxyl-terminal hydrolase BAP1 Recombinant Protein

货号:

R14117h

商标:

EIAab^®

监管等级:

RUO

别名:

BRCA1-associated protein 1, Cerebral protein 6, hucep-6, KIAA0272

序列号:

Q92560

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

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Human BAP1 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 3p21.1 by HGNC 3p21.1 by Entrez Gene 3p21.1 by Ensembl

BAP1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Component of the PR-DUB complex, at least composed of BAP1 and ASXL1. Interacts with BRCA1 (via the RING finger). Interacts (via HBM-like motif) with HCFC1.

功能:

Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed:24703950). Acts as a tumor suppressor.

亚细胞位置:

Cytoplasm Nucleus Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O (PubMed:24703950).

数据库链接

UniGene:

Hs.106674

SMR:

Q92560

STRING:

9606.ENSP00000417132

KEGG:

hsa:8314

MIM:

156240

Pfam:

PF01088

Uniprot:

Q92560

相关产品

Mouse Bap1 ELISA Kit

Rat Bap1 ELISA Kit

Bovine BAP1 ELISA Kit

Chicken BAP1 ELISA Kit

Mouse Bap1 CLIA Kit

序列长度:

729

序列:

仅供参考，详细请咨询（services@eiaab.com）

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729:

RQGVSIGRLH | KQRKPDRRKR | SRPYKAKRQ

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression."

(PMID: 9528852)

[2].

"Genetic sequence variations of BRCA1-interacting genes AURKA, BAP1, BARD1 and DHX9 in French Canadian families with high risk of breast cancer."

(PMID: 19197335)

[3].

"BRCA1-associated protein 1 interferes with BRCA1/BARD1 RING heterodimer activity."

(PMID: 19117993)

[4].

"The deubiquitinating enzyme BAP1 regulates cell growth via interaction with HCF-1."

(PMID: 19815555)

[5].

"Novel tumor antigens identified by autologous antibody screening of childhood medulloblastoma cDNA libraries."

(PMID: 12800201)

[6].

"Germline BAP1 mutations predispose also to multiple basal cell carcinomas."

(PMID: 25080371)

[7].

"Autodeubiquitination protects the tumor suppressor BAP1 from cytoplasmic sequestration mediated by the atypical ubiquitin ligase UBE2O."

(PMID: 24703950)

[8].

"Germline BAP1 mutations predispose to malignant mesothelioma."

(PMID: 21874000)

[9].

"The nuclear deubiquitinase BAP1 is commonly inactivated by somatic mutations and 3p21.1 losses in malignant pleural mesothelioma."

(PMID: 21642991)

[10].

"Germline mutations in BAP1 predispose to melanocytic tumors."

(PMID: 21874003)

[1].

人BAP1重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人BAP1重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人BAP1重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人BAP1重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人BAP1重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人BAP1重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人BAP1重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人BAP1重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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