首页 > 重组蛋白 > Human ATR Recombinant Protein

ATR (基因名), Serine/threonine-protein kinase ATR (蛋白名), atr_human.

产品名称:

Human ATR/ Serine/threonine-protein kinase ATR Recombinant Protein
丝氨酸/苏氨酸蛋白激酶ATR

货号:

R15117h

商标:

EIAab^®

监管等级:

RUO

别名:

Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1, FRP1

序列号:

Q13535

来源:

E.coli

种属:

Human

标签:

His

序列:

2296-2644

预估分子量:

38.39 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

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Human ATR Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human ATR protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 3q23 by HGNC 3q23 by Entrez Gene 3q23 by Ensembl

ATR Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with ATRIP; forming a heterodimer with ATRIP (PubMed:11721054). Binds to DNA, and to UV-damaged DNA with higher affinity. Interacts with RAD17, MSH2 and HDAC2. Present in a complex containing ATRIP and RPA-coated single-stranded DNA. Present in a complex containing CHD4 and HDAC2. Interacts with BCR-ABL after genotoxic stress. Interacts with EEF1E1. This interaction is enhanced by UV irradiation. Interacts with CLSPN and CEP164. Interacts with TELO2 and TTI1.

功能:

Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.

亚细胞位置:

Nucleus Nucleus PML body Chromosome Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.

数据库链接

UniGene:

Hs.271791

STRING:

9606.ENSP00000343741

KEGG:

hsa:545

MIM:

210600

Pfam:

PF02259

Uniprot:

Q13535

相关产品

Mouse Atr ELISA Kit

Mouse Atr CLIA Kit

Mouse Atr Protein

Mouse Atr Polyclonal Antibody

Mouse Atr Monoclonal Antibody

序列长度:

2644

序列:

仅供参考，详细请咨询（services@eiaab.com）

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2644:

GVIKTRNRVT | GLPLSIEGHV | HYLIQEATDE | NLLCQMYLGW | TPYM

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"cDNA cloning and gene mapping of a candidate human cell cycle checkpoint protein."

(PMID: 8610130)

[2].

"ATR mutation in endometrioid endometrial cancer is associated with poor clinical outcomes."

(PMID: 19470935)

[3].

"Protein phosphatase 5 is required for ATR-mediated checkpoint activation."

(PMID: 16260606)

[4].

"Mutation analysis of the ATR gene in breast and ovarian cancer families."

(PMID: 15987455)

[5].

"Seckel syndrome exhibits cellular features demonstrating defects in the ATR-signalling pathway."

(PMID: 15496423)

[1].

人丝氨酸/苏氨酸蛋白激酶ATR(ATR)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人丝氨酸/苏氨酸蛋白激酶ATR(ATR)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人丝氨酸/苏氨酸蛋白激酶ATR(ATR)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人丝氨酸/苏氨酸蛋白激酶ATR(ATR)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人丝氨酸/苏氨酸蛋白激酶ATR(ATR)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人丝氨酸/苏氨酸蛋白激酶ATR(ATR)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人丝氨酸/苏氨酸蛋白激酶ATR(ATR)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人丝氨酸/苏氨酸蛋白激酶ATR(ATR)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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