首页 > 重组蛋白 > Human ADA Recombinant Protein

ADA (基因名), Adenosine deaminase (蛋白名), ada_human.

产品名称:

Human ADA/ Adenosine deaminase Recombinant Protein
腺苷脱氨

货号:

R1390h

商标:

EIAab^®

监管等级:

RUO

别名:

Adenosine aminohydrolase, ADA1

序列号:

P00813

来源:

E.coli

种属:

Human

标签:

His

序列:

2-363aa

预估分子量:

39.82 kDa (monomer)

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Epigenetics

规格 & 价格:

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Human ADA Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 20q13.12 by HGNC 20q13.12 by Entrez Gene 20q13.12 by Ensembl

ADA Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with DPP4 (via extracellular domain) (PubMed:10951221, PubMed:14691230, PubMed:7907293, PubMed:8101391, PubMed:15016824). Interacts with PLG (via Kringle 4 domain); the interaction stimulates PLG activation when in complex with DPP4 (PubMed:15016824).

功能:

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine (PubMed:8452534, PubMed:16670267). Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4 (PubMed:20959412). Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (PubMed:11772392). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation (PubMed:20959412). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (PubMed:23193172). Stimulates plasminogen activation (PubMed:15016824). Plays a role in male fertility (PubMed:21919946, PubMed:26166670). Plays a protective role in early postimplantation embryonic development.

亚细胞位置:

Cell membrane Peripheral membrane protein Extracellular side Cell junction Cytoplasmic vesicle lumen Cytoplasm Lysosome Colocalized with DPP4 at the cell surface.

数据库链接

UniGene:

Hs.654536

SMR:

P00813

STRING:

9606.ENSP00000361965

KEGG:

hsa:100

MIM:

102700

Pfam:

PF00962

Uniprot:

P00813

相关产品

Mouse Ada ELISA Kit

Rat Ada ELISA Kit

Bovine ADA ELISA Kit

Chicken ADA ELISA Kit

Mouse Ada CLIA Kit

序列长度:

363

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

363:

YGMPPSASAG | QNL

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Endothelial catabolism of extracellular adenosine during hypoxia: the role of surface adenosine deaminase and CD26."

(PMID: 16670267)

[2].

"Genetic predisposition of responsiveness to therapy for chronic hepatitis C."

(PMID: 16886895)

[3].

"A functional genetic variation of adenosine deaminase affects the duration and intensity of deep sleep in humans."

(PMID: 16221767)

[4].

"Cell surface adenosine deaminase binds and stimulates plasminogen activation on 1-LN human prostate cancer cells."

(PMID: 15016824)

[5].

"Adenosine deaminase alleles and autistic disorder: case-control and family-based association studies."

(PMID: 11121182)

[6].

"Two newly identified mutations (Thr233Ile and Leu152Met) in partially adenosine deaminase-deficient (ADA-) individuals that result in differing biochemical and metabolic phenotypes."

(PMID: 9225964)

[7].

"An adenosine deaminase (ADA) allele contains two newly identified deleterious mutations (Y97C and L106V) that interact to abolish enzyme activity."

(PMID: 9361033)

[8].

"Three new adenosine deaminase mutations that define a splicing enhancer and cause severe and partial phenotypes: implications for evolution of a CpG hotspot and expression of a transduced ADA cDNA."

(PMID: 8589684)

[9].

"Demonstration of adenosine deaminase activity in human fibroblast lysosomes."

(PMID: 8452534)

[10].

"Human adenosine deaminase. cDNA and complete primary amino acid sequence."

(PMID: 6090454)

[1].

人腺苷脱氨(ADA)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人腺苷脱氨(ADA)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人腺苷脱氨(ADA)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人腺苷脱氨(ADA)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人腺苷脱氨(ADA)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人腺苷脱氨(ADA)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人腺苷脱氨(ADA)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人腺苷脱氨(ADA)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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