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Trpv1 (基因名), Transient receptor potential cation channel subfamily V member 1 (蛋白名), trpv1_mouse.
产品名称:

Mouse Trpv1/ Transient receptor potential cation channel subfamily V member 1 ELISA Kit
瞬态电压感受器阳离子通道亚科V成员1

货号:

E12152m

商标:
EIAab®
监管等级:
别名:

Osm-9-like TRP channel 1, Vanilloid receptor 1, OTRPC1, TrpV1

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.156-10ng/mL
灵敏度:
0.078ng/mL
特异性:
Natural and recombinant mouse Transient receptor potential cation channel subfamily V member 1
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Signal Transduction
Mouse Trpv1 ELISA Kit
规格 & 价格: cart
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Mouse Trpv1 ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤5.8%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤8.3%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

86

80-92

Plasma

88

82-94

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

103-112%

83-93%

89-99%

95-104%

EDTA plasma(n=5)

103-111%

100-100%

97-107%

117-127%

heparin plasma(n=5)

105-114%

 

101-111%

106-119%

91-101%

 

通用注释


亚单元:
Homotetramer. May also form a heteromeric channel with TRPV3 (By similarity). Interacts with CALM, PRKCM and CSK (By similarity). Interacts with PRKCG and NTRK1, probably by forming a trimeric complex (By similarity). Interacts with PIRT (PubMed:18455988). Interacts with TMEM100 (PubMed:25640077).


功能:
Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli (PubMed:15194687, PubMed:15489017). Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia (PubMed:10764638). Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis.


亚细胞位置:
Cell junction Synapse Postsynaptic cell membrane Multi-pass membrane protein Cell projection Dendritic spine membrane Multi-pass membrane protein Cell membrane Multi-pass membrane protein Mostly, but not exclusively expressed in postsynaptic dendritic spines.


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