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Trpm2 (基因名), Transient receptor potential cation channel subfamily M member 2 (蛋白名), trpm2_mouse.

产品名称:

Mouse Trpm2/ Transient receptor potential cation channel subfamily M member 2 ELISA Kit
瞬时受体电位阳离子通道亚家族M成员2

货号:

E12145m

商标:

EIAab^®

监管等级:

RUO

别名:

Long transient receptor potential channel 2, Transient receptor potential channel 7, LTrpC-2, TrpC7, Ltrpc2, Trpc7

检测方法:

ELISA

特异性:

Natural and recombinant mouse Transient receptor potential cation channel subfamily M member 2

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

Cardiovascular

规格 & 价格:

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常见问题解答
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通用注释

亚单元:

N/A

功能:

Nonselective, voltage-independent cation channel that mediates Na(+) and Ca(2+) influx, leading to increased cytoplasmic Ca(2+) levels (PubMed:11804595, PubMed:19454650, PubMed:21753080, PubMed:22493272). Extracellular calcium passes through the channel and increases channel activity by binding to the cytoplasmic domain and stabilizing the channel in an open conformation (By similarity). Also contributes to Ca(2+) release from intracellular stores in response to ADP-ribose (PubMed:21753080). Plays a role in numerous processes that involve signaling via intracellular Ca(2+) levels (PubMed:21753080). Besides, mediates the release of lysosomal Zn(2+) stores in response to reactive oxygen species, leading to increased cytosolic Zn(2+) levels (By similarity). Activated by moderate heat (35 to 40 degrees Celsius) (PubMed:27533035, PubMed:27562954). Activated by intracellular ADP-ribose, beta-NAD (NAD(+)) and similar compounds, and by oxidative stress caused by reactive oxygen or nitrogen species (PubMed:19454650, PubMed:21753080, PubMed:22493272). The precise physiological activators are under debate; the true, physiological activators may be ADP-ribose and ADP-ribose-2'-phosphate. Activation by ADP-ribose and beta-NAD is strongly increased by moderate heat (35 to 40 degrees Celsius) (By similarity). Likewise, reactive oxygen species lower the threshold for activation by moderate heat (37 degrees Celsius) (PubMed:22493272, PubMed:25817999). Plays a role in mediating behavorial and physiological responses to moderate heat and thereby contributes to body temperature homeostasis (PubMed:27533035, PubMed:27562954). Plays a role in insulin secretion, a process that requires increased cytoplasmic Ca(2+) levels (PubMed:20921208, PubMed:25817999). Required for normal IFNG and cytokine secretion and normal innate immune immunity in response to bacterial infection (PubMed:21709234). Required for normal phagocytosis and cytokine release by macrophages exposed to zymosan (in vitro) (PubMed:22493272). Plays a role in dendritic cell differentiation and maturation, and in dendritic cell chemotaxis via its role in regulating cytoplasmic Ca(2+) levels (PubMed:21753080). Plays a role in the regulation of the reorganization of the actin cytoskeleton and filopodia formation in response to reactive oxygen species via its function in increasing cytoplasmic Ca(2+) and Zn(2+) levels (By similarity). Confers susceptibility to cell death following oxidative stress (PubMed:25562606).

亚细胞位置:

Cell membrane Multi-pass membrane protein Perikaryon Cell projection Cytoplasmic vesicle Lysosome Detected at the cell membrane and in intracellular vesicles in cortical neurons. Detected on neuronal cell bodies and neurites (By similarity). Detected on the cell membrane in polymorphonuclear neutrophils (PubMed:21753080). Detected on cytoplasmic vesicles and lysosomes in immature bone marrow dendritic cells (PubMed:21753080).

数据库链接

UniGene:

Mm.276762

STRING:

10090.ENSMUSP00000101040

KEGG:

mmu:28240

Pfam:

PF00520

Uniprot:

Q91YD4

相关产品

Human TRPM2 ELISA Kit

序列长度:

1506

序列:

仅供参考，详细请咨询（services@eiaab.com）

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LFGAHF

3D 模型:

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免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

小鼠瞬时受体电位阳离子通道亚家族M成员2(Trpm2)ELISA试剂盒可以做多少个样本？

小鼠瞬时受体电位阳离子通道亚家族M成员2(Trpm2)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠瞬时受体电位阳离子通道亚家族M成员2(Trpm2)ELISA试剂盒使用视频？

小鼠瞬时受体电位阳离子通道亚家族M成员2(Trpm2)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠瞬时受体电位阳离子通道亚家族M成员2(Trpm2)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠瞬时受体电位阳离子通道亚家族M成员2(Trpm2)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠瞬时受体电位阳离子通道亚家族M成员2(Trpm2)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠瞬时受体电位阳离子通道亚家族M成员2(Trpm2)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠瞬时受体电位阳离子通道亚家族M成员2(Trpm2)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠瞬时受体电位阳离子通道亚家族M成员2(Trpm2)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠瞬时受体电位阳离子通道亚家族M成员2(Trpm2)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠瞬时受体电位阳离子通道亚家族M成员2(Trpm2)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠瞬时受体电位阳离子通道亚家族M成员2(Trpm2)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠瞬时受体电位阳离子通道亚家族M成员2(Trpm2)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Mouse Trpm2/ Transient receptor potential cation channel subfamily M member 2 ELISA Kit 瞬时受体电位阳离子通道亚家族M成员2

E12145m

Long transient receptor potential channel 2, Transient receptor potential channel 7, LTrpC-2, TrpC7, Ltrpc2, Trpc7

Mouse Trpm2/ Transient receptor potential cation channel subfamily M member 2 ELISA Kit
瞬时受体电位阳离子通道亚家族M成员2