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Zfp36l2 (基因名), mRNA decay activator protein ZFP36L2 (蛋白名), tisd_mouse.

产品名称:

Mouse Zfp36l2/ mRNA decay activator protein ZFP36L2 ELISA Kit
mRNA衰变激活蛋白ZFP36L2

货号:

E5956m

商标:

EIAab^®

监管等级:

RUO

别名:

Butyrate response factor 2, TPA-induced sequence 11d, Zinc finger protein 36, C3H1 type-like 2, ZFP36-like 2, Brf2, Tis11d

检测方法:

ELISA

特异性:

Natural and recombinant mouse mRNA decay activator protein ZFP36L2

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

规格 & 价格:

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Mouse Zfp36l2 ELISA Kit

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数据
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序列 / 3D
常见问题解答
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通用注释

亚单元:

Associates with the cytoplasmic CCR4-NOT deadenylase to trigger ARE-containing mRNA deadenylation and decay processes. Interacts with CNOT7; this interaction is inhibited in response to phorbol 12-myristate 13-acetate (PMA) treatment in a p38 MAPK-dependent manner.

功能:

Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis (PubMed:22701344, PubMed:22367205, PubMed:25505318, PubMed:24830504, PubMed:27102483). Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery (By similarity). Functions by recruiting the CCR4-NOT deadenylase complex and probably other components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes (By similarity). Binds to 3'-UTR ARE of numerous mRNAs (PubMed:22701344, PubMed:22367205, PubMed:25505318, PubMed:24830504). Promotes ARE-containing mRNA decay of the low-density lipoprotein (LDL) receptor (LDLR) mRNA in response to phorbol 12-myristate 13-acetate (PMA) treatment in a p38 MAPK-dependent manner (By similarity). Positively regulates early adipogenesis by promoting ARE-mediated mRNA decay of immediate early genes (IEGs) (PubMed:22701344). Plays a role in mature peripheral neuron integrity by promoting ARE-containing mRNA decay of the transcriptional repressor REST mRNA (PubMed:25505318). Plays a role in ovulation and oocyte meiotic maturation by promoting ARE-mediated mRNA decay of the luteinizing hormone receptor LHCGR mRNA (PubMed:24830504). Acts as a negative regulator of erythroid cell differentiation: promotes glucocorticoid-induced self-renewal of erythroid cells by binding mRNAs that are induced or highly expressed during terminal erythroid differentiation and promotes their degradation, preventing erythroid cell differentiation (PubMed:19633199, PubMed:23748442). In association with ZFP36L1 maintains quiescence on developing B lymphocytes by promoting ARE-mediated decay of several mRNAs encoding cell cycle regulators that help B cells progress through the cell cycle, and hence ensuring accurate variable-diversity-joining (VDJ) recombination process and functional immune cell formation (PubMed:27102483). Together with ZFP36L1 is also necessary for thymocyte development and prevention of T-cell acute lymphoblastic leukemia (T-ALL) transformation by promoting ARE-mediated mRNA decay of the oncogenic transcription factor NOTCH1 mRNA (PubMed:20622884).

亚细胞位置:

Nucleus Cytoplasm Shuttles between the nucleus and the cytoplasm in a XPO1/CRM1-dependent manner (PubMed:11796723, PubMed:22367205).

数据库链接

UniGene:

Mm.259321

SMR:

P23949

STRING:

10090.ENSMUSP00000050820

KEGG:

mmu:12193

Pfam:

PF04553

Uniprot:

P23949

相关产品

Human ZFP36L2 ELISA Kit

Human ZFP36L2 CLIA Kit

Human ZFP36L2 Protein

Human ZFP36L2 Polyclonal Antibody

Human ZFP36L2 Monoclonal Antibody

序列长度:

484

序列:

仅供参考，详细请咨询（services@eiaab.com）

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484:

SGSLSSGSLS | GSESPSLDPG | RRLPIFSRLS | ISDD

3D 模型:

3D 结构:

免疫原序列:

验证序列:

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[1].

小鼠mRNA衰变激活蛋白ZFP36L2(Zfp36l2)ELISA试剂盒可以做多少个样本？

小鼠mRNA衰变激活蛋白ZFP36L2(Zfp36l2)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠mRNA衰变激活蛋白ZFP36L2(Zfp36l2)ELISA试剂盒使用视频？

小鼠mRNA衰变激活蛋白ZFP36L2(Zfp36l2)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠mRNA衰变激活蛋白ZFP36L2(Zfp36l2)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠mRNA衰变激活蛋白ZFP36L2(Zfp36l2)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠mRNA衰变激活蛋白ZFP36L2(Zfp36l2)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠mRNA衰变激活蛋白ZFP36L2(Zfp36l2)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠mRNA衰变激活蛋白ZFP36L2(Zfp36l2)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠mRNA衰变激活蛋白ZFP36L2(Zfp36l2)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠mRNA衰变激活蛋白ZFP36L2(Zfp36l2)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠mRNA衰变激活蛋白ZFP36L2(Zfp36l2)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠mRNA衰变激活蛋白ZFP36L2(Zfp36l2)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠mRNA衰变激活蛋白ZFP36L2(Zfp36l2)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Mouse Zfp36l2/ mRNA decay activator protein ZFP36L2 ELISA Kit mRNA衰变激活蛋白ZFP36L2

E5956m

Butyrate response factor 2, TPA-induced sequence 11d, Zinc finger protein 36, C3H1 type-like 2, ZFP36-like 2, Brf2, Tis11d

Mouse Zfp36l2/ mRNA decay activator protein ZFP36L2 ELISA Kit
mRNA衰变激活蛋白ZFP36L2