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Qtrt1 (基因名), Queuine tRNA-ribosyltransferase catalytic subunit 1 (蛋白名), tgt_mouse.
产品名称:

Mouse Qtrt1/ Queuine tRNA-ribosyltransferase catalytic subunit 1 ELISA Kit

货号:
-
商标:
EIAab®
监管等级:
别名:

Guanine insertion enzyme, tRNA-guanine transglycosylase, Tgt, Tgut

检测方法:
ELISA
特异性:
Natural and recombinant mouse Queuine tRNA-ribosyltransferase catalytic subunit 1
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
研究领域:
-
Mouse Qtrt1 ELISA Kit
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Mouse Qtrt1 ELISA Kit
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通用注释


亚单元:
Heterodimer of a catalytic subunit QTRT1 and an accessory subunit QTRT2.


功能:
Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:19414587). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product.


亚细胞位置:
Cytoplasm Mitochondrion outer membrane Peripheral membrane protein Cytoplasmic side Nucleus Weakly associates with mitochondria, possibly via QTRT2.


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