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TET1 (基因名), Methylcytosine dioxygenase TET1 (蛋白名), tet1_human.

产品名称:

Human TET1/ Methylcytosine dioxygenase TET1 ELISA Kit
甲基胞嘧啶tet1双加氧酶

货号:

E14778h

商标:

EIAab^®

监管等级:

RUO

别名:

CXXC-type zinc finger protein 6, Leukemia-associated protein with a CXXC domain, Ten-eleven translocation 1 gene protein, 1.14.11.n2, CXXC6, KIAA1676, LCX

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.156-10ng/mL

灵敏度:

0.069ng/mL

特异性:

Natural and recombinant human Methylcytosine dioxygenase TET1

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

规格 & 价格:

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产品说明书

数据表:

说明书:

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常见问题解答
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亚单元:

Interacts with HCFC1 and OGT (By similarity). Interacts with SIN3A; recruits the transcriptional corepressor SIN3A to gene promoters (PubMed:21490601). Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1 (By similarity).

功能:

Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Plays an important role in the tumorigenicity of glioblastoma cells. TET1-mediated production of 5hmC acts as a recruitment signal for the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).

亚细胞位置:

Nucleus

数据库链接

UniGene:

Hs.258855

SMR:

Q8NFU7

STRING:

9606.ENSP00000362748

KEGG:

hsa:80312

MIM:

607790

Pfam:

PF12851

Uniprot:

Q8NFU7

相关产品

Mouse Tet1 ELISA Kit

Mouse Tet1 CLIA Kit

Mouse Tet1 Protein

Mouse Tet1 Polyclonal Antibody

Mouse Tet1 Monoclonal Antibody

序列长度:

2136

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NVVTVSPYAL | THVAGPYNHW | V

3D 模型:

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免疫原序列:

验证序列:

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[1].

人甲基胞嘧啶tet1双加氧酶(TET1)ELISA试剂盒可以做多少个样本？

人甲基胞嘧啶tet1双加氧酶(TET1)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人甲基胞嘧啶tet1双加氧酶(TET1)ELISA试剂盒使用视频？

人甲基胞嘧啶tet1双加氧酶(TET1)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人甲基胞嘧啶tet1双加氧酶(TET1)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人甲基胞嘧啶tet1双加氧酶(TET1)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人甲基胞嘧啶tet1双加氧酶(TET1)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人甲基胞嘧啶tet1双加氧酶(TET1)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人甲基胞嘧啶tet1双加氧酶(TET1)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人甲基胞嘧啶tet1双加氧酶(TET1)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人甲基胞嘧啶tet1双加氧酶(TET1)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人甲基胞嘧啶tet1双加氧酶(TET1)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人甲基胞嘧啶tet1双加氧酶(TET1)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人甲基胞嘧啶tet1双加氧酶(TET1)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Human TET1/ Methylcytosine dioxygenase TET1 ELISA Kit 甲基胞嘧啶tet1双加氧酶

E14778h

CXXC-type zinc finger protein 6, Leukemia-associated protein with a CXXC domain, Ten-eleven translocation 1 gene protein, 1.14.11.n2, CXXC6, KIAA1676, LCX

Human TET1/ Methylcytosine dioxygenase TET1 ELISA Kit
甲基胞嘧啶tet1双加氧酶