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TAOK2 (基因名), Serine/threonine-protein kinase TAO2 (蛋白名), taok2_human.

产品名称:

Human TAOK2/ Serine/threonine-protein kinase TAO2 ELISA Kit
丝氨酸/ threonine-protein激酶TAO2

货号:

E2239h

商标:

EIAab^®

监管等级:

RUO

别名:

Kinase from chicken homolog C, Prostate-derived sterile 20-like kinase 1, Thousand and one amino acid protein kinase 2, hKFC-C, PSK-1, UNQ2971/PRO7431, KIAA0881, MAP3K17, PSK, PSK1

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.156-10ug/mL

灵敏度:

0.078ug/mL

特异性:

Natural and recombinant human Serine/threonine-protein kinase TAO2

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

规格 & 价格:

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产品说明书

说明书:

MSDS:

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数据
文献
序列 / 3D
常见问题解答
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通用注释

亚单元:

Interacts with MAP2K3 and MAP2K6 (By similarity). Self-associates. Interacts with tubulins through the C-terminal domain. Interacts with MAP3K7 and interfers with MAP3K7-binding to CHUK and thus prevents NF-kappa-B activation. Isoform 2 interacts with PCDH8; this complex may also include CDH2.

功能:

Serine/threonine-protein kinase involved in different processes such as membrane blebbing and apoptotic bodies formation DNA damage response and MAPK14/p38 MAPK stress-activated MAPK cascade. Phosphorylates itself, MBP, activated MAPK8, MAP2K3, MAP2K6 and tubulins. Activates the MAPK14/p38 MAPK signaling pathway through the specific activation and phosphorylation of the upstream MAP2K3 and MAP2K6 kinases. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Isoform 1, but not isoform 2, plays a role in apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation. This function, which requires the activation of MAPK8/JNK and nuclear localization of C-terminally truncated isoform 1, may be linked to the mitochondrial CASP9-associated death pathway. Isoform 1 binds to microtubules and affects their organization and stability independently of its kinase activity. Prevents MAP3K7-mediated activation of CHUK, and thus NF-kappa-B activation, but not that of MAPK8/JNK. May play a role in the osmotic stress-MAPK8 pathway. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization. Both isoforms are involved in MAPK14 phosphorylation.

亚细胞位置:

Isoform 2 Cell projection Dendrite In dendrites, colocalizes with PCDH8.

数据库链接

UniGene:

Hs.291623

SMR:

Q9UL54

STRING:

9606.ENSP00000310094

KEGG:

hsa:9344

MIM:

613199

Pfam:

PF00069

Uniprot:

Q9UL54

相关产品

Mouse Taok2 ELISA Kit

序列长度:

1235

序列:

仅供参考，详细请咨询（services@eiaab.com）

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1235:

SQRQLGPPAS | RQPLPGTLAG | RRSRTRQSRA | LPPWR

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

人丝氨酸/ threonine-protein激酶TAO2(TAOK2)ELISA试剂盒可以做多少个样本？

人丝氨酸/ threonine-protein激酶TAO2(TAOK2)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人丝氨酸/ threonine-protein激酶TAO2(TAOK2)ELISA试剂盒使用视频？

人丝氨酸/ threonine-protein激酶TAO2(TAOK2)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人丝氨酸/ threonine-protein激酶TAO2(TAOK2)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人丝氨酸/ threonine-protein激酶TAO2(TAOK2)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人丝氨酸/ threonine-protein激酶TAO2(TAOK2)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人丝氨酸/ threonine-protein激酶TAO2(TAOK2)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人丝氨酸/ threonine-protein激酶TAO2(TAOK2)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人丝氨酸/ threonine-protein激酶TAO2(TAOK2)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人丝氨酸/ threonine-protein激酶TAO2(TAOK2)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人丝氨酸/ threonine-protein激酶TAO2(TAOK2)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人丝氨酸/ threonine-protein激酶TAO2(TAOK2)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人丝氨酸/ threonine-protein激酶TAO2(TAOK2)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Human TAOK2/ Serine/threonine-protein kinase TAO2 ELISA Kit 丝氨酸/ threonine-protein激酶TAO2

E2239h

Kinase from chicken homolog C, Prostate-derived sterile 20-like kinase 1, Thousand and one amino acid protein kinase 2, hKFC-C, PSK-1, UNQ2971/PRO7431, KIAA0881, MAP3K17, PSK, PSK1

Human TAOK2/ Serine/threonine-protein kinase TAO2 ELISA Kit
丝氨酸/ threonine-protein激酶TAO2