GH1 (基因名), Somatotropin (蛋白名), soma_pig.
产品名称:
Pig GH1/ Somatotropin ELISA Kit
生长激素
货号:
E0044p
商标:
EIAab®
监管等级:
别名:
Growth hormone
检测方法:
ELISA
实验类型:
Competitive
检测范围:
0.312-20ng/mL
灵敏度:
0.095ng/mL
特异性:
Natural and recombinant pig Somatotropin
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
登录.
实验步骤:
研究领域:
Signal Transduction
精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤4.5%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤7.3%
批内 CV: ≤4.5%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤7.3%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。
Recovery was determined by spiking various levels of GH1 into serum and plasma .
Sample Type | Average(%) | Recovery Range(%) |
Serum | 108 | 105-110 |
Plasma | 109 | 107-112 |
线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 86-96% | 90-100% | 107-118% | 104-112% |
EDTA plasma(n=5) | 95-104% | 91-100% | 98-108% | 102-112% |
heparin plasma(n=5) | 105-114%
| 111-121% | 103-113% | 80-90% |
通用注释
亚单元:
N/A
功能:
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
亚细胞位置:
Secreted
该产品尚未在任何出版物中被引用。
[1].
猪生长激素(GH1)ELISA试剂盒可以做多少个样本?
猪生长激素(GH1)ELISA试剂盒分为2种规格,96孔和48孔。96孔的试剂盒,标曲和样本都做复孔的话,可以检测40个样本。96孔的试剂盒,标曲和样本都不做复孔的话,可以检测88个样本。
[2].
猪生长激素(GH1)ELISA试剂盒使用视频?
猪生长激素(GH1)ELISA试剂盒实验操作视频在以下网址中,对每一步的实验步骤都做了演示,方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html
https://www.eiaab.com.cn/lesson-tech/805.html
[3].
猪生长激素(GH1)ELISA试剂盒是放在-20℃冰箱保存吗?
EIAab的猪生长激素(GH1)ELISA试剂盒,洗涤液、底物、终止液保存于4℃,其余试剂-20℃冰箱保存。
[4].
猪生长激素(GH1)ELISA试剂盒原理?
双抗体夹心法:用纯化的抗体包被微孔板,制成固相抗体,往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
竞争法:用纯化的抗体包被微孔板,制成固相抗体,往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物,受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
竞争法:用纯化的抗体包被微孔板,制成固相抗体,往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物,受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
[5].
猪生长激素(GH1)ELISA试剂盒中需要使用的样品量是多少?
夹心法100μL/孔,竞争法50μL/孔。如样本浓度过高时,应对样本进行稀释,以使稀释后的样本符合试剂盒的检测范围,计算时再乘以相应的稀释倍数。
[6].
如何分析猪生长激素(GH1)ELISA试剂盒数据?
建议标准曲线,并计算样本浓度。对于elisa的曲线拟合,一般建议采用4参数曲线拟合,4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合,例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度,再乘以稀释倍数;或用标准物的浓度与OD值计算出标曲的回归方程式,将样本的OD值代入方程式,计算出样本浓度,再乘以稀释倍数,即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/
https://www.eiaab.com.cn/news/502/
[7].
猪生长激素(GH1)ELISA试剂盒中是否包含人和动物的副产物,是否包含感染的或者传染性原料如HIV等?
除了抗体和稀释液中的BSA,不含其它人和动物的副产物,也不含感染材料。
[8].
收集猪生长激素(GH1)ELISA试剂盒血浆样本,用什么作为抗凝剂?
一般建议用EDTA和肝素作为抗凝剂。
[9].
猪生长激素(GH1)ELISA试剂盒酶标板可以拆成几部分?拆的时候是否需要避光,无菌?
猪生长激素(GH1)ELISA试剂盒酶标板是8×12孔条,可拆卸,板子可以拆成12条,注意避免孔污染,不需要避光和无菌。暂时不用的板子,放回原来装的袋子里,密封保存。
[10].
猪生长激素(GH1)ELISA试剂盒样本如何保存?
尽量检测新鲜样本。若无新鲜样本,则4℃保存1周,-20℃保存1个月,-80℃保存2个月。
反馈墙
评论数 : 1
所有用户
所有用户
customer
默认排序
默认排序
最近
早期
E0044P -Porcine Growth Hormone ELISA Kit
Our customers had the following comments on this kit can you respond to this please.
Thanks for attaching the protocol for this elisa. Is this also called pig C4a. In this elisa (if it is against pig growth hormone?), do you have more specific information about the antibody used (what epitope it recognizes or what has been used as immunogen)?
Also the typical standard curve shown in the protocol, is that really relevant for this assay? Although I am not experienced in running competitive binding elisas, as I understand it you get and increased signal at lower concentrations compared to higher concentrations of your sample?
http://www.eiaab.com/entries/steps/ELISA%20Kit/SOMA_PIG/Pig
thank you for your help with this
customer
2015-01-04 00:00
2015-01-04 00:00
Pig C4a is another ELISA kit Cat. E0389p and this item is for Pig Somatotropin. I can confirm that the antibodies used in these kits have been raised against recombinant protein and purified proteins. The exact epitope of the antibodies have not been investigated further.
The standard curve on the protocol is one of the results just for reference and you should do new standare curve for every test.
In a competitive ELISA the competitor is labeled with biotin and the amount of the bound competitor is measured. This means that a high OD indicates a low amount of the protein of interest in your sample. A low OD means that the competitor has been ousted and there is a high amount of the protein of interest in the sample.
Please let me know if there is anything else that we can do for you, and I'll be happy to help.
star
2015-01-04 17:57
2015-01-04 17:57
0
0
通知
规格
数量
单价 (¥)
小计 1 (¥)
小计 2:
¥
规格
数量
单价 (¥)