首页 > 酶联免疫试剂盒 > Rat Shtn1 ELISA Kit

Shtn1 (基因名), Shootin-1 (蛋白名), shot1_rat.

产品名称:

Rat Shtn1/ Shootin-1 ELISA Kit

货号:

E3842r

商标:

EIAab^®

监管等级:

RUO

别名:

Shootin1

检测方法:

ELISA

特异性:

Natural and recombinant rat Shootin-1

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

规格 & 价格:

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通用注释

亚单元:

Interacts with PFN2. Interacts (via N-terminus) with KIF20B; this interaction is direct and promotes the association of SHTN1 to microtubules in primary neurons. Associates with microtubule (By similarity). Interacts with L1CAM; this interaction occurs in axonal growth cones (PubMed:18519736). Interacts with actin filament retrograde flow; this interaction is enhanced in a netrin-1- and PAK1-dependent manner and promotes F-actin-substrate coupling and concomitant formation of traction forces at axonal growth cones (PubMed:18519736, PubMed:23453953). Interacts with RUFY3 (PubMed:17030985).

功能:

Involved in the generation of internal asymmetric signals required for neuronal polarization and neurite outgrowth (PubMed:17030985, PubMed:17439943, PubMed:18519736, PubMed:20664640). Mediates netrin-1-induced F-actin-substrate coupling or 'clutch engagement' within the axon growth cone through activation of CDC42, RAC1 and PAK1-dependent signaling pathway, thereby converting the F-actin retrograde flow into traction forces, concomitantly with filopodium extension and axon outgrowth (PubMed:18519736, PubMed:23453953). Plays a role in cytoskeletal organization by regulating the subcellular localization of phosphoinositide 3-kinase (PI3K) activity at the axonal growth cone (PubMed:17030985). Plays also a role in regenerative neurite outgrowth (PubMed:20664640). In the developing cortex, cooperates with KIF20B to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in the accumulation of phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the growth cone of primary hippocampal neurons.

亚细胞位置:

Perikaryon Cell projection Axon Cell projection Growth cone Cytoplasm Cytoskeleton Cell projection Filopodium Cell projection Lamellipodium Localizes in multiple growth cones at neurite tips before the neuronal symmetry-breaking step (PubMed:23453953). Accumulates in growth cones of a single nascent axon in a neurite length-dependent manner during the neuronal symmetry-breaking step; when absent from the nascent axon's siblings, probably due to competitive transport, prevents the formation of surplus axons (PubMed:17030985, PubMed:23453953). Transported anterogradely from the soma to the axon growth cone in an actin and myosin-dependent manner and passively diffuses back to the cell bodies (PubMed:23453953). Colocalized with L1CAM in close apposition with actin filaments in filopodia and lamellipodia of axonal growth cones in hippocampal neurons (PubMed:18519736). Exhibits retrograde movements in filopodia and lamellopodia of axonal growth cones (PubMed:18519736). Colocalized with KIF20B along microtubules to the tip of the growing cone in primary hippocampal neurons. Recruted to the growth cone of developing axon in a KIF20B- and microtubule-dependent manner (By similarity).

数据库链接

UniGene:

Rn.16095

SMR:

A0MZ67

STRING:

10116.ENSRNOP00000061843

KEGG:

rno:292139

Uniprot:

A0MZ67

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序列长度:

633

序列:

仅供参考，详细请咨询（services@eiaab.com）

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633:

PTQCKEEERG | ETQPEFKEDS | SGGKTGETDS | SNC

3D 模型:

3D 结构:

免疫原序列:

验证序列:

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[1].

大鼠Shtn1ELISA试剂盒可以做多少个样本？

大鼠Shtn1ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

大鼠Shtn1ELISA试剂盒使用视频？

大鼠Shtn1ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

大鼠Shtn1ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的大鼠Shtn1ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

大鼠Shtn1ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

大鼠Shtn1ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析大鼠Shtn1ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

大鼠Shtn1ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集大鼠Shtn1ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

大鼠Shtn1ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

大鼠Shtn1ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

大鼠Shtn1ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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