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Rara (基因名), Retinoic acid receptor alpha (蛋白名), rara_mouse.

产品名称:

Mouse Rara/ Retinoic acid receptor alpha ELISA Kit
视黄酸受体-alpha

货号:

E2297m

商标:

EIAab^®

监管等级:

RUO

别名:

Nuclear receptor subfamily 1 group B member 1, RAR-alpha, Nr1b1

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.312-20ng/mL

灵敏度:

0.168ng/mL

特异性:

Natural and recombinant mouse Retinoic acid receptor alpha

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Cancer

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

数据
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序列 / 3D
常见问题解答
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通用注释

亚单元:

Interacts with PRMT2 (By similarity). Interacts with LRIF1 (By similarity). Interacts with NCOA7 in a ligand-inducible manner. Interacts with KMT2E/MLL5. Interacts (via the ligand-binding domain) with PRAME; interaction is direct and ligand (retinoic acid)-dependent. Interacts with PRKAR1A; the interaction negatively. regulates RARA transcriptional activity. Interacts with NCOR1 and NCOR2; the interaction occurs in the absence of ligand and represses transcriptional activity. Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with CDK7; the interaction is enhanced by interaction with GTF2H3. Interacts with GTF2H3; the interaction requires prior phosphorylation on Ser-369 which then enhances interaction with CDK7. Interacts with ASXL1 and NCOA1. Interacts with ACTN4.

功能:

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.

亚细胞位置:

Nucleus Cytoplasm Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus is dependent on activation of PKC and the downstream MAPK phosphorylation.

数据库链接

UniGene:

Mm.439744

SMR:

P11416

STRING:

10090.ENSMUSP00000069744

KEGG:

mmu:19401

Pfam:

PF00104

Uniprot:

P11416

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Dog RARA ELISA Kit

Human RARA CLIA Kit

Chicken RARA CLIA Kit

序列长度:

462

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

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462:

SSHRSSPATQ | SP

3D 模型:

3D 结构:

免疫原序列:

验证序列:

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[1].

小鼠视黄酸受体-alpha(Rara)ELISA试剂盒可以做多少个样本？

小鼠视黄酸受体-alpha(Rara)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠视黄酸受体-alpha(Rara)ELISA试剂盒使用视频？

小鼠视黄酸受体-alpha(Rara)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠视黄酸受体-alpha(Rara)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠视黄酸受体-alpha(Rara)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠视黄酸受体-alpha(Rara)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠视黄酸受体-alpha(Rara)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠视黄酸受体-alpha(Rara)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠视黄酸受体-alpha(Rara)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠视黄酸受体-alpha(Rara)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠视黄酸受体-alpha(Rara)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠视黄酸受体-alpha(Rara)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠视黄酸受体-alpha(Rara)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Mouse Rara/ Retinoic acid receptor alpha ELISA Kit 视黄酸受体-alpha

E2297m

Nuclear receptor subfamily 1 group B member 1, RAR-alpha, Nr1b1

Mouse Rara/ Retinoic acid receptor alpha ELISA Kit
视黄酸受体-alpha