RAB7A (基因名), Ras-related protein Rab-7a (蛋白名), rab7a_human.
产品名称:
Human RAB7A/ Ras-related protein Rab-7a ELISA Kit
Ras-relatedproteinRab-7a
货号:
E14686h
商标:
EIAab®
监管等级:
别名:
RAB7
检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.78-50ng/mL
灵敏度:
0.23ng/mL
特异性:
Natural and recombinant human Ras-related protein Rab-7a
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
登录.
实验步骤:
研究领域:
-
线性
线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。
Sample | 1：2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 96-104% | 107-119% | 107-117% | 107-117% |
EDTA plasma(n=5) | 99-108% | 100-112% | 91-103% | 106-115% |
heparin plasma(n=5) | 97-107%
| 87-98% | 86-99% | 111-111% |
通用注释
亚单元:
The GTP-bound form interacts with RAC1 (By similarity). Interacts with NTRK1/TRKA (By similarity). Interacts with C9orf72 (By similarity). Interacts with CHM, the substrate-binding subunit of the Rab geranylgeranyltransferase complex (By similarity). Interacts with RILP (PubMed:11179213, PubMed:14668488, PubMed:15933719, PubMed:20028791, PubMed:22431521). Interacts with PSMA7 (PubMed:14998988). Interacts with RNF115 (PubMed:16925951). Interacts with FYCO1 (PubMed:20100911). Interacts with the PIK3C3/VPS34-PIK3R4 complex (PubMed:14617358). The GTP-bound form interacts with OSBPL1A (PubMed:16176980). Interacts with CLN3 (PubMed:22261744). Does not interact with HPS4 and the BLOC-3 complex (heterodimer of HPS1 and HPS4) (PubMed:20048159). Interacts with CLN5 (PubMed:22431521).
功能:
Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA (PubMed:11179213, PubMed:12944476, PubMed:14617358, PubMed:20028791, PubMed:21255211). Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (By similarity). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
亚细胞位置:
Cytoplasmic vesicle
Phagosome membrane
Peripheral membrane protein
Cytoplasmic side
Late endosome membrane
Peripheral membrane protein
Cytoplasmic side
Lysosome membrane
Peripheral membrane protein
Cytoplasmic side
Melanosome membrane
Peripheral membrane protein
Cytoplasmic side
Cytoplasmic vesicle
Autophagosome membrane
Peripheral membrane protein
Cytoplasmic side
Lipid droplet
Endosome membrane
Colocalizes with OSBPL1A at the late endosome (PubMed:16176980). Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium (PubMed:21255211). Lipid droplet localization is increased upon ADRB2 stimulation (By similarity).
数据库链接
验证序列:
该产品尚未在任何出版物中被引用。
[1].
人Ras-relatedproteinRab-7a(RAB7A)ELISA试剂盒可以做多少个样本？
人Ras-relatedproteinRab-7a(RAB7A)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。
[2].
人Ras-relatedproteinRab-7a(RAB7A)ELISA试剂盒使用视频？
人Ras-relatedproteinRab-7a(RAB7A)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html
https://www.eiaab.com.cn/lesson-tech/805.html
[3].
人Ras-relatedproteinRab-7a(RAB7A)ELISA试剂盒是放在-20℃冰箱保存吗？
EIAab的人Ras-relatedproteinRab-7a(RAB7A)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。
[4].
人Ras-relatedproteinRab-7a(RAB7A)ELISA试剂盒原理？
双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
[5].
人Ras-relatedproteinRab-7a(RAB7A)ELISA试剂盒中需要使用的样品量是多少？
夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。
[6].
如何分析人Ras-relatedproteinRab-7a(RAB7A)ELISA试剂盒数据？
建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/
https://www.eiaab.com.cn/news/502/
[7].
人Ras-relatedproteinRab-7a(RAB7A)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？
除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。
[8].
收集人Ras-relatedproteinRab-7a(RAB7A)ELISA试剂盒血浆样本，用什么作为抗凝剂？
一般建议用EDTA和肝素作为抗凝剂。
[9].
人Ras-relatedproteinRab-7a(RAB7A)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？
人Ras-relatedproteinRab-7a(RAB7A)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。
[10].
人Ras-relatedproteinRab-7a(RAB7A)ELISA试剂盒样本如何保存？
尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。
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