首页 > 酶联免疫试剂盒 > Rat Rab10 ELISA Kit

Rab10 (基因名), Ras-related protein Rab-10 (蛋白名), rab10_rat.

产品名称:

Rat Rab10/ Ras-related protein Rab-10 ELISA Kit
Ras相关蛋白Rab-10

货号:

E5608r

商标:

EIAab^®

监管等级:

RUO

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.156-10ng/mL

灵敏度:

0.081ng/mL

特异性:

Natural and recombinant rat Ras-related protein Rab-10

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Signal Transduction

规格 & 价格:

关注

Rat Rab10 ELISA Kit

邮箱 *

消息 *

Please 登录.

产品说明书

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
反馈墙 (0)

通用注释

亚单元:

Interacts (GDP-bound form) with LLGL1; the interaction is direct and promotes RAB10 association with membranes and activation through competition with the Rab inhibitor GDI1. Interacts (GTP-bound form) with MICALCL, MICAL1, MICAL3, EHBP1 and EHBP1L1; at least in case of MICAL1 two molecules of RAB10 can bind to one molecule of MICAL1.

功能:

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. Finally, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.

亚细胞位置:

Cytoplasmic vesicle membrane Lipid-anchor Cytoplasmic side Golgi apparatus Trans-Golgi network membrane Endosome membrane Recycling endosome membrane Cytoplasmic vesicle Phagosome membrane Cell projection Cilium Endoplasmic reticulum membrane Associates with SLC2A4/GLUT4 storage vesicles (By similarity). Localizes to the base of the cilium (PubMed:20576682). Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (By similarity).

数据库链接

UniGene:

Rn.65864

SMR:

P35281

STRING:

10116.ENSRNOP00000065234

Pfam:

PF00071

Uniprot:

P35281

相关产品

Human RAB10 ELISA Kit

Mouse Rab10 ELISA Kit

Chicken RAB10 ELISA Kit

Dog RAB10 ELISA Kit

Human RAB10 CLIA Kit

序列长度:

200

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

大鼠Ras相关蛋白Rab-10(Rab10)ELISA试剂盒可以做多少个样本？

大鼠Ras相关蛋白Rab-10(Rab10)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

大鼠Ras相关蛋白Rab-10(Rab10)ELISA试剂盒使用视频？

大鼠Ras相关蛋白Rab-10(Rab10)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

大鼠Ras相关蛋白Rab-10(Rab10)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的大鼠Ras相关蛋白Rab-10(Rab10)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

大鼠Ras相关蛋白Rab-10(Rab10)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

大鼠Ras相关蛋白Rab-10(Rab10)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析大鼠Ras相关蛋白Rab-10(Rab10)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

大鼠Ras相关蛋白Rab-10(Rab10)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集大鼠Ras相关蛋白Rab-10(Rab10)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

大鼠Ras相关蛋白Rab-10(Rab10)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

大鼠Ras相关蛋白Rab-10(Rab10)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

大鼠Ras相关蛋白Rab-10(Rab10)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

反馈墙

评论数 : 0

所有用户

默认排序

Rat Rab10/ Ras-related protein Rab-10 ELISA Kit Ras相关蛋白Rab-10

E5608r

Rat Rab10/ Ras-related protein Rab-10 ELISA Kit
Ras相关蛋白Rab-10