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Nlrp1b (基因名), NACHT, LRR and PYD domains-containing protein 1b allele 1 (蛋白名), nl1b1_mouse.

产品名称:

Mouse Nlrp1b/ NACHT, LRR and PYD domains-containing protein 1b allele 1 ELISA Kit

货号:

E15885m

商标:

EIAab^®

监管等级:

RUO

别名:

Nalp1b

检测方法:

ELISA

特异性:

Natural and recombinant mouse NACHT, LRR and PYD domains-containing protein 1b allele 1

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

规格 & 价格:

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Mouse Nlrp1b ELISA Kit

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通用注释

亚单元:

Sensor component of NLRP1 inflammasomes. Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation. Classical inflammasomes consist of a signal sensor component, an adapter (ASC/PYCARD), which recruits an effector proinflammatory caspase (CASP1 and possibly CASP4/CASP11). CASP1 filament formation increases local enzyme concentration, resulting in trans-autocleavage and activation. Active CASP1 then processes IL1B and IL18 precursors, leading to the release of mature cytokines in the extracellular milieu and inflammatory response. In NLRP1 inflammasome, the role of PYCARD is not clear. Following activation, Nlrp1b may directly interact with CASP1 (through the CARD domain) to form a functional inflammasome (PubMed:19124602, PubMed:19651869, PubMed:24492532). Hence PYCARD may not be necessary for NLRP1 and CASP1 interaction, but is required for speck formation and full inflammasome activity (PubMed:19651869, PubMed:24492532). Homomer (PubMed:19651869, PubMed:22536155). Interacts (via LRR repeats) with BCL2 and BCL2L1 (via the loop between motifs BH4 and BH3); these interactions reduce NLRP1 inflammasome-induced CASP1 activation and IL1B release, possibly by impairing NLRP1 interaction with PYCARD (By similarity). Interacts with NOD2; this interaction may increase IL1B release (By similarity). Interacts with EIF2AK2/PKR; this interaction requires EIF2AK2 activity, is accompanied by EIF2AK2 autophosphorylation and promotes inflammasome assembly in response to Bacillus anthracis lethal toxin (PubMed:22801494). Interacts with MEFV; this interaction targets NLRP1 to degradation by autophagy, hence preventing excessive IL1B- and IL18-mediated inflammation.

功能:

As the sensor component of the NLRP1 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of Nlrp1b, CASP1, and possibly PYCARD. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP1 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PubMed:19651869, PubMed:21170303, PubMed:22753929, PubMed:22536155, PubMed:23818853). Activated by cleavage by Bacillus anthracis lethal toxin (LT) endopeptidase component (PubMed:19124602, PubMed:19651869, PubMed:19949100, PubMed:22536155, PubMed:23818853,PubMed:24935976, PubMed:24492532). Activated by metabolic inhibitors, such as 2-deoxy-D-glucose and sodium azide, by nutrient deprivation and hypoxia, possibly due to a decrease in cytosolic ATP (PubMed:23230290, PubMed:24935976). Also activated by Toxoplasma gondii (PubMed:24218483). Not activated by muramyl dipeptide, nor by full-length bacterial peptidoglycan (PubMed:22753929). Primary mediator of macrophage susceptibility to LT. In response to Bacillus anthracis infection, macrophages and dendritic cells release IL1B and undergo pyroptosis, an inflammatory form of programmed cell death. This early inflammatory response to the toxin increases resistance to infection by B. anthracis spores (PubMed:16429160, PubMed:19949100, PubMed:21170303, PubMed:22753929, PubMed:23818853). Binds ATP.

亚细胞位置:

Cytoplasm Cytoplasm Cytosol Inflammasome Membrane

数据库链接

UniGene:

Mm.390402

Pfam:

PF00619

Uniprot:

Q2LKW6

相关产品

Mouse Nlrp1b CLIA Kit

序列长度:

1233

序列:

仅供参考，详细请咨询（services@eiaab.com）

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RACKDLFYQA | LKETHPHLVM | DLLEKSGGVS | LGS

3D 模型:

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免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

小鼠Nlrp1bELISA试剂盒可以做多少个样本？

小鼠Nlrp1bELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠Nlrp1bELISA试剂盒使用视频？

小鼠Nlrp1bELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠Nlrp1bELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠Nlrp1bELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠Nlrp1bELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠Nlrp1bELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠Nlrp1bELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠Nlrp1bELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠Nlrp1bELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠Nlrp1bELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠Nlrp1bELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠Nlrp1bELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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