首页 > 酶联免疫试剂盒 > Human NFE2L1 ELISA Kit

NFE2L1 (基因名), Endoplasmic reticulum membrane sensor NFE2L1 (蛋白名), nf2l1_human.

产品名称:

Human NFE2L1/ Endoplasmic reticulum membrane sensor NFE2L1 ELISA Kit
核因子E2相关因子1

货号:

E15027h

商标:

EIAab^®

监管等级:

RUO

别名:

Locus control region-factor 1, Nuclear factor erythroid 2-related factor 1, Nuclear factor, erythroid derived 2, like 1, Protein NRF1, p120 form, Transcription factor 11, LCR-F1, NF-E2-related factor 1, TCF-11, HBZ17, NRF1, TCF11

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

78-5000pg/mL

灵敏度:

35pg/ml

特异性:

Natural and recombinant human Endoplasmic reticulum membrane sensor NFE2L1

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Epigenetics

规格 & 价格:

关注

Human NFE2L1 ELISA Kit

邮箱 *

消息 *

Please 登录.

产品说明书

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
反馈墙 (0)

精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤6.3%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤9.9%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	103	97-109
Plasma	105	99-111

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	101-109%	98-109%	90-102%	103-113%
EDTA plasma(n=5)	104-114%	105-115%	103-115%	107-117%
heparin plasma(n=5)	108-118%	108-117%	101-110%	109-119%

通用注释

亚单元:

Interacts with KEAP1 (PubMed:16687406). Endoplasmic reticulum membrane sensor NFE2L1: Interacts (via CPD region) with FBXW7; leading to its ubiquitination and degradation (By similarity). Endoplasmic reticulum membrane sensor NFE2L1: Interacts with SYVN1/HRD1; leading to its ubiquitination and degradation (By similarity). Endoplasmic reticulum membrane sensor NFE2L1: Interacts (when ubiquitinated) with DDI2; leading to its cleavage (By similarity). Transcription factor NRF1: Interacts (via the bZIP domain) with small MAF protein (MAFF, MAFG or MAFK); required for binding to antioxidant response elements (AREs) on DNA (PubMed:8932385, PubMed:9421508). Transcription factor NRF1: Interacts (via Destruction motif) with BTRC; leading to its ubiquitination and degradation (By similarity). Transcription factor NRF1: Interacts with CEBPB; the heterodimer represses expression of DSPP during odontoblast differentiation (PubMed:15308669).

功能:

Transcription factor NRF1: CNC-type bZIP family transcription factor that translocates to the nucleus and regulates expression of target genes in response to various stresses (PubMed:8932385, PubMed:9421508). Heterodimerizes with small-Maf proteins (MAFF, MAFG or MAFK) and binds DNA motifs including the antioxidant response elements (AREs), which regulate expression of genes involved in oxidative stress response (PubMed:8932385, PubMed:9421508). Activates or represses expression of target genes, depending on the context (PubMed:8932385, PubMed:9421508). Plays a key role in cholesterol homeostasis by acting as a sensor of cholesterol excess: in low cholesterol conditions, translocates into the nucleus and represses expression of genes involved in defense against cholesterol excess, such as CD36 (By similarity). In excess cholesterol conditions, the endoplasmic reticulum membrane form of the protein directly binds cholesterol via its CRAC motif, preventing cleavage and release of the transcription factor NRF1, thereby allowing expression of genes promoting cholesterol removal (By similarity). Critical for redox balance in response to oxidative stress: acts by binding the AREs motifs on promoters and mediating activation of oxidative stress response genes, such as GCLC, GCLM, GSS, MT1 and MT2 (By similarity). Plays an essential role during fetal liver hematopoiesis: probably has a protective function against oxidative stress and is involved in lipid homeostasis in the liver (By similarity). Involved in proteasome homeostasis: in response to proteasome inhibition, mediates the 'bounce-back' of proteasome subunits by translocating into the nucleus and activating expression of genes encoding proteasome subunits (PubMed:20932482). Also involved in regulating glucose flux (By similarity). Together with CEBPB; represses expression of DSPP during odontoblast differentiation (PubMed:15308669). In response to ascorbic acid induction, activates expression of SP7/Osterix in osteoblasts.

亚细胞位置:

Transcription factor NRF1 Nucleus Translocates into the nucleus following cleavage of Endoplasmic reticulum membrane sensor NFE2L1 by aspartyl protease DDI2.

数据库链接

UniGene:

Hs.514284

SMR:

Q14494

STRING:

9606.ENSP00000354855

KEGG:

hsa:4779

MIM:

163260

Pfam:

PF03131

Uniprot:

Q14494

相关产品

Mouse Nfe2l1 ELISA Kit

Bovine NFE2L1 ELISA Kit

Chicken NFE2L1 ELISA Kit

Mouse Nfe2l1 CLIA Kit

Bovine NFE2L1 CLIA Kit

序列长度:

772

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

784:

ALQYAGDGSV | LLIPRTMADQ | QARRQERKPK | DRRK

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

人核因子E2相关因子1(NFE2L1)ELISA试剂盒可以做多少个样本？

人核因子E2相关因子1(NFE2L1)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人核因子E2相关因子1(NFE2L1)ELISA试剂盒使用视频？

人核因子E2相关因子1(NFE2L1)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人核因子E2相关因子1(NFE2L1)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人核因子E2相关因子1(NFE2L1)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人核因子E2相关因子1(NFE2L1)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人核因子E2相关因子1(NFE2L1)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人核因子E2相关因子1(NFE2L1)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人核因子E2相关因子1(NFE2L1)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人核因子E2相关因子1(NFE2L1)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人核因子E2相关因子1(NFE2L1)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人核因子E2相关因子1(NFE2L1)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人核因子E2相关因子1(NFE2L1)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

反馈墙

评论数 : 0

所有用户

默认排序

Human NFE2L1/ Endoplasmic reticulum membrane sensor NFE2L1 ELISA Kit 核因子E2相关因子1

E15027h

Locus control region-factor 1, Nuclear factor erythroid 2-related factor 1, Nuclear factor, erythroid derived 2, like 1, Protein NRF1, p120 form, Transcription factor 11, LCR-F1, NF-E2-related factor 1, TCF-11, HBZ17, NRF1, TCF11

Human NFE2L1/ Endoplasmic reticulum membrane sensor NFE2L1 ELISA Kit
核因子E2相关因子1