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Mettl3 (基因名), N6-adenosine-methyltransferase subunit METTL3 (蛋白名), mta70_mouse.

产品名称:

Mouse Mettl3/ N6-adenosine-methyltransferase subunit METTL3 ELISA Kit
N6-adenosine-methyltransferase催化亚基

货号:

E13478m

商标:

EIAab^®

监管等级:

RUO

别名:

Methyltransferase-like protein 3, N6-adenosine-methyltransferase 70 kDa subunit, MT-A70, Mta70

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.156-10ng/mL

灵敏度:

0.039ng/mL

特异性:

Natural and recombinant mouse N6-adenosine-methyltransferase subunit METTL3

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

规格 & 价格:

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产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
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序列 / 3D
常见问题解答
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通用注释

亚单元:

Heterodimer; heterodimerizes with METTL14 to form an antiparallel heterodimer that constitutes an active methyltransferase. Component of the WMM complex, a N6-methyltransferase complex composed of WTAP, METTL3 and METTL14. Interacts with NCBP1/CBP80. Interacts with EIF4E. Interacts with EIF3B.

功能:

The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of some RNAs and regulates the circadian clock, differentiation of embryonic stem cells and primary miRNA processing (PubMed:25456834, PubMed:24394384, PubMed:25569111). In the heterodimer formed with METTL14, METTL3 constitutes the catalytic core (By similarity). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability (By similarity). M6A regulates the length of the circadian clock: acts as an early pace-setter in the circadian loop by putting mRNA production on a fast-track for facilitating nuclear processing, thereby providing an early point of control in setting the dynamics of the feedback loop (PubMed:24209618). M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization, promoting differentiation of ESCs (PubMed:25456834, PubMed:24394384, PubMed:25569111). M6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs) (By similarity). METTL3 also mediates methylation of pri-miRNAs, marking them for recognition and processing by DGCR8 (By similarity). Acts as a positive regulator of mRNA translation independently of the methyltransferase activity: promotes translation by interacting with the translation initiation machinery in the cytoplasm.

亚细胞位置:

Nucleus Nucleus speckle Cytoplasm Colocalizes with speckles in interphase nuclei. Suggesting that it may be associated with nuclear pre-mRNA splicing components (PubMed:24394384).

数据库链接

UniGene:

Mm.271759

SMR:

Q8C3P7

STRING:

10090.ENSMUSP00000022767

KEGG:

mmu:56335

Pfam:

PF05063

Uniprot:

Q8C3P7

相关产品

Human METTL3 ELISA Kit

Human METTL3 CLIA Kit

Human METTL3 Protein

Human METTL3 Polyclonal Antibody

Human METTL3 Monoclonal Antibody

序列长度:

580

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

580:

LDGIHLLDPD | VVARFKQRYP | DGIISKPKNL |

3D 模型:

3D 结构:

免疫原序列:

验证序列:

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[1].

小鼠N6-adenosine-methyltransferase催化亚基(Mettl3)ELISA试剂盒可以做多少个样本？

小鼠N6-adenosine-methyltransferase催化亚基(Mettl3)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠N6-adenosine-methyltransferase催化亚基(Mettl3)ELISA试剂盒使用视频？

小鼠N6-adenosine-methyltransferase催化亚基(Mettl3)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠N6-adenosine-methyltransferase催化亚基(Mettl3)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠N6-adenosine-methyltransferase催化亚基(Mettl3)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠N6-adenosine-methyltransferase催化亚基(Mettl3)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠N6-adenosine-methyltransferase催化亚基(Mettl3)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠N6-adenosine-methyltransferase催化亚基(Mettl3)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠N6-adenosine-methyltransferase催化亚基(Mettl3)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠N6-adenosine-methyltransferase催化亚基(Mettl3)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠N6-adenosine-methyltransferase催化亚基(Mettl3)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠N6-adenosine-methyltransferase催化亚基(Mettl3)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠N6-adenosine-methyltransferase催化亚基(Mettl3)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Mouse Mettl3/ N6-adenosine-methyltransferase subunit METTL3 ELISA Kit N6-adenosine-methyltransferase催化亚基

E13478m

Methyltransferase-like protein 3, N6-adenosine-methyltransferase 70 kDa subunit, MT-A70, Mta70

Mouse Mettl3/ N6-adenosine-methyltransferase subunit METTL3 ELISA Kit
N6-adenosine-methyltransferase催化亚基