首页 > 酶联免疫试剂盒 > Human MAP2K1 ELISA Kit

MAP2K1 (基因名), Dual specificity mitogen-activated protein kinase kinase 1 (蛋白名), mp2k1_human.

产品名称:

Human MAP2K1/ Dual specificity mitogen-activated protein kinase kinase 1 ELISA Kit
双重特异性促分裂原活化蛋白激酶激酶1

货号:

E10995h

商标:

EIAab^®

监管等级:

RUO

别名:

ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP kinase kinase 1, MEK1, PRKMK1

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.156-10ng/mL

灵敏度:

0.078ng/mL

特异性:

Natural and recombinant human Dual specificity mitogen-activated protein kinase kinase 1

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Cancer

规格 & 价格:

关注

Human MAP2K1 ELISA Kit

邮箱 *

消息 *

Please 登录.

产品说明书

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
反馈墙 (0)

精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤4.6%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤8.3%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	113	107-119
Plasma	115	109-120

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	87-95%	82-92%	108-118%	97-108%
EDTA plasma(n=5)	81-93%	110-120%	106-115%	102-111%
heparin plasma(n=5)	93-102%	103-113%	84-96%	105-115%

通用注释

亚单元:

Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3/ERK1 and RGS14. Forms a heterodimer with MAP2K2/MEK2. Forms heterodimers with KSR2 which further dimerize to form tetramers. Interacts with ARBB2, LAMTOR3, MAPK1/ERK2, MORG1 and RAF1 (By similarity). Interacts with PPARG and with isoform 1 of VRK2 (PubMed:20679487, PubMed:17101779). Interacts with Yersinia yopJ (PubMed:16728640). Interacts with SGK1 (PubMed:19447520). Interacts with BIRC6/bruce (PubMed:18329369). Interacts with KSR1 (PubMed:10409742).

功能:

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

亚细胞位置:

Cytoplasm Cytoskeleton Microtubule organizing center Centrosome Cytoplasm Cytoskeleton Microtubule organizing center Spindle pole body Cytoplasm Nucleus Membrane Peripheral membrane protein Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis (PubMed:14737111). Membrane localization is probably regulated by its interaction with KSR1 (PubMed:10409742).

数据库链接

UniGene:

Hs.145442

SMR:

Q02750

STRING:

9606.ENSP00000302486

KEGG:

hsa:5604

MIM:

176872

Pfam:

PF00069

Uniprot:

Q02750

相关产品

Mouse Map2k1 ELISA Kit

Rat Map2k1 ELISA Kit

Mouse Map2k1 CLIA Kit

Rat Map2k1 CLIA Kit

Mouse Map2k1 Protein

序列长度:

393

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

393:

DLKQLMVHAF | IKRSDAEEVD | FAGWLCSTIG | LNQPSTPTHA | AGV

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

人双重特异性促分裂原活化蛋白激酶激酶1(MAP2K1)ELISA试剂盒可以做多少个样本？

人双重特异性促分裂原活化蛋白激酶激酶1(MAP2K1)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人双重特异性促分裂原活化蛋白激酶激酶1(MAP2K1)ELISA试剂盒使用视频？

人双重特异性促分裂原活化蛋白激酶激酶1(MAP2K1)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人双重特异性促分裂原活化蛋白激酶激酶1(MAP2K1)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人双重特异性促分裂原活化蛋白激酶激酶1(MAP2K1)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人双重特异性促分裂原活化蛋白激酶激酶1(MAP2K1)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人双重特异性促分裂原活化蛋白激酶激酶1(MAP2K1)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人双重特异性促分裂原活化蛋白激酶激酶1(MAP2K1)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人双重特异性促分裂原活化蛋白激酶激酶1(MAP2K1)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人双重特异性促分裂原活化蛋白激酶激酶1(MAP2K1)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人双重特异性促分裂原活化蛋白激酶激酶1(MAP2K1)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人双重特异性促分裂原活化蛋白激酶激酶1(MAP2K1)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人双重特异性促分裂原活化蛋白激酶激酶1(MAP2K1)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

反馈墙

评论数 : 0

所有用户

默认排序

Human MAP2K1/ Dual specificity mitogen-activated protein kinase kinase 1 ELISA Kit 双重特异性促分裂原活化蛋白激酶激酶1

E10995h

ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP kinase kinase 1, MEK1, PRKMK1

Human MAP2K1/ Dual specificity mitogen-activated protein kinase kinase 1 ELISA Kit
双重特异性促分裂原活化蛋白激酶激酶1